Specific Trans-Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses
Synaptic transmission depends on the matching and alignment of presynaptically released transmitters and postsynaptic neurotransmitter receptors. Neuroligin (NL) and Neurexin (Nrxn) proteins are trans-synaptic adhesion molecules that are important in validation and maturation of specific synapses. N...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en_US |
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Society for Neuroscience
2013
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| Online Access: | http://hdl.handle.net/1721.1/80785 |
| _version_ | 1826192653980532736 |
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| author | Futai, Kensuke Doty, Christopher D. Baek, Brian Ryu, Jubin Sheng, Morgan Hwa-Tze |
| author2 | Picower Institute for Learning and Memory |
| author_facet | Picower Institute for Learning and Memory Futai, Kensuke Doty, Christopher D. Baek, Brian Ryu, Jubin Sheng, Morgan Hwa-Tze |
| author_sort | Futai, Kensuke |
| collection | MIT |
| description | Synaptic transmission depends on the matching and alignment of presynaptically released transmitters and postsynaptic neurotransmitter receptors. Neuroligin (NL) and Neurexin (Nrxn) proteins are trans-synaptic adhesion molecules that are important in validation and maturation of specific synapses. NL isoforms NL1 and NL2 have specific functional roles in excitatory and inhibitory synapses, respectively, but the molecular basis behind this distinction is still unclear. We show here that the extracellular domain of NL2 confers its unique ability to enhance inhibitory synaptic function when overexpressed in rat hippocampal pyramidal neurons, whereas NL1 normally only promotes excitatory synapses. This specificity is conferred by presynaptic Nrxn isoforms, as NL1 can also induce functional inhibitory synapse connections when the presynaptic interneurons ectopically express an Nrxn isoform that binds to NL1. Our results indicate that trans-synaptic interaction with differentially expressed presynaptic Nrxns underlies the distinct functions of NL1 and NL2, and is sufficient to induce functional inhibitory synapse formation. |
| first_indexed | 2024-09-23T09:26:31Z |
| format | Article |
| id | mit-1721.1/80785 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:26:31Z |
| publishDate | 2013 |
| publisher | Society for Neuroscience |
| record_format | dspace |
| spelling | mit-1721.1/807852022-09-26T11:27:02Z Specific Trans-Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses Futai, Kensuke Doty, Christopher D. Baek, Brian Ryu, Jubin Sheng, Morgan Hwa-Tze Picower Institute for Learning and Memory Futai, Kensuke Baek, Brian Ryu, Jubin Sheng, Morgan Hwa-Tze Synaptic transmission depends on the matching and alignment of presynaptically released transmitters and postsynaptic neurotransmitter receptors. Neuroligin (NL) and Neurexin (Nrxn) proteins are trans-synaptic adhesion molecules that are important in validation and maturation of specific synapses. NL isoforms NL1 and NL2 have specific functional roles in excitatory and inhibitory synapses, respectively, but the molecular basis behind this distinction is still unclear. We show here that the extracellular domain of NL2 confers its unique ability to enhance inhibitory synaptic function when overexpressed in rat hippocampal pyramidal neurons, whereas NL1 normally only promotes excitatory synapses. This specificity is conferred by presynaptic Nrxn isoforms, as NL1 can also induce functional inhibitory synapse connections when the presynaptic interneurons ectopically express an Nrxn isoform that binds to NL1. Our results indicate that trans-synaptic interaction with differentially expressed presynaptic Nrxns underlies the distinct functions of NL1 and NL2, and is sufficient to induce functional inhibitory synapse formation. University of Massachusetts (System) {Start-up Funds) Whitehall Foundation 2013-09-18T13:50:32Z 2013-09-18T13:50:32Z 2013-02 2012-12 Article http://purl.org/eprint/type/JournalArticle 0270-6474 1529-2401 http://hdl.handle.net/1721.1/80785 Futai, K., C. D. Doty, B. Baek, J. Ryu, and M. Sheng. “Specific Trans-Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses.” Journal of Neuroscience 33, no. 8 (February 20, 2013): 3612-3623. en_US http://dx.doi.org/10.1523/jneurosci.1811-12.2013 Journal of Neuroscience Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Society for Neuroscience Society for Neuroscience |
| spellingShingle | Futai, Kensuke Doty, Christopher D. Baek, Brian Ryu, Jubin Sheng, Morgan Hwa-Tze Specific Trans-Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses |
| title | Specific Trans-Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses |
| title_full | Specific Trans-Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses |
| title_fullStr | Specific Trans-Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses |
| title_full_unstemmed | Specific Trans-Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses |
| title_short | Specific Trans-Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses |
| title_sort | specific trans synaptic interaction with inhibitory interneuronal neurexin underlies differential ability of neuroligins to induce functional inhibitory synapses |
| url | http://hdl.handle.net/1721.1/80785 |
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