Imaging Protein–Protein Interactions inside Living Cells via Interaction-Dependent Fluorophore Ligation

Imaging Protein–Protein Interactions inside Living Cells via Interaction-Dependent Fluorophore Ligation

We report a new method, Interaction-Dependent PRobe Incorporation Mediated by Enzymes, or ID-PRIME, for imaging protein–protein interactions (PPIs) inside living cells. ID-PRIME utilizes a mutant of Escherichia coli lipoic acid ligase, LplA[superscript W37V], which can catalyze the covalent ligation...

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Bibliographic Details
Main Authors: Slavoff, Sarah A., Liu, Daniel S., Cohen, Justin D., Ting, Alice Y.
Other Authors: Massachusetts Institute of Technology. Department of Chemistry
Format: Article
Language:en_US
Published: American Chemical Society (ACS) 2013
Online Access:http://hdl.handle.net/1721.1/82558
https://orcid.org/0000-0002-8277-5226

Internet

http://hdl.handle.net/1721.1/82558
https://orcid.org/0000-0002-8277-5226

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