Thermal Stabilization of an Endoglucanase by Cyclization

An intein-driven protein splicing approach allowed for the covalent linkage between the N- and C-termini of a polypeptide chain to create circular variants of the endo-β-1,3-1,4-glucanase, LicA, from Bacillus licheniformis. Two circular variants, LicA-C1 and LicA-C2, which have connecting loops of 2...

Descripció completa

Dades bibliogràfiques
Autors principals: Lieshout, Johan F. T., Vroom, Wietse, Planas, Antoni, Vos, Willem M., Oost, John, Koutsopoulos, Sotirios, Gutierrez, Odette N. Perez, Kouusopoulos, Sotiros
Altres autors: Massachusetts Institute of Technology. Center for Biomedical Engineering
Format: Article
Idioma:en_US
Publicat: Springer-Verlag 2013
Accés en línia:http://hdl.handle.net/1721.1/82660