Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs

Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs

Most metazoan microRNAs (miRNAs) target many genes for repression, but the nematode lsy-6 miRNA is much less proficient. Here we show that the low proficiency of lsy-6 can be recapitulated in HeLa cells and that miR-23, a mammalian miRNA, also has low proficiency in these cells. Reporter results and...

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Bibliographic Details
Main Authors: Garcia, David M., Baek, Daehyun, Shin, Chanseok, Bell, George W., Grimson, Andrew, Bartel, David
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Nature Publishing Group 2013
Online Access:http://hdl.handle.net/1721.1/82943
https://orcid.org/0000-0002-3872-2856

Internet

http://hdl.handle.net/1721.1/82943
https://orcid.org/0000-0002-3872-2856

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