Negative Regulation of Fibroblast Motility by Ena/VASP Proteins
Ena/VASP proteins have been implicated in cell motility through regulation of the actin cytoskeleton and are found at focal adhesions and the leading edge. Using overexpression, loss-of-function, and inhibitory approaches, we find that Ena/VASP proteins negatively regulate fibroblast motility. A dos...
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| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/83484 https://orcid.org/0000-0003-3214-4554 |
| _version_ | 1811085625512689664 |
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| author | Libova, Irina Bear, James E. Loureiro, Joseph J. Gertler, Frank Fassler, Reinhard Wehland, Jurgen |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Libova, Irina Bear, James E. Loureiro, Joseph J. Gertler, Frank Fassler, Reinhard Wehland, Jurgen |
| author_sort | Libova, Irina |
| collection | MIT |
| description | Ena/VASP proteins have been implicated in cell motility through regulation of the actin cytoskeleton and are found at focal adhesions and the leading edge. Using overexpression, loss-of-function, and inhibitory approaches, we find that Ena/VASP proteins negatively regulate fibroblast motility. A dose-dependent decrease in movement is observed when Ena/VASP proteins are overexpressed in fibroblasts. Neutralization or deletion of all Ena/VASP proteins results in increased cell movement. Selective depletion of Ena/VASP proteins from focal adhesions, but not the leading edge, has no effect on motility. Constitutive membrane targeting of Ena/VASP proteins inhibits motility. These results are in marked contrast to current models for Ena/VASP function derived mainly from their role in the actin-driven movement of Listeria monocytogenes. |
| first_indexed | 2024-09-23T13:12:32Z |
| format | Article |
| id | mit-1721.1/83484 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:12:32Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/834842022-09-28T12:39:07Z Negative Regulation of Fibroblast Motility by Ena/VASP Proteins Libova, Irina Bear, James E. Loureiro, Joseph J. Gertler, Frank Fassler, Reinhard Wehland, Jurgen Massachusetts Institute of Technology. Department of Biology Bear, James E. Loureiro, Joseph J. Libova, Irina Gertler, Frank Ena/VASP proteins have been implicated in cell motility through regulation of the actin cytoskeleton and are found at focal adhesions and the leading edge. Using overexpression, loss-of-function, and inhibitory approaches, we find that Ena/VASP proteins negatively regulate fibroblast motility. A dose-dependent decrease in movement is observed when Ena/VASP proteins are overexpressed in fibroblasts. Neutralization or deletion of all Ena/VASP proteins results in increased cell movement. Selective depletion of Ena/VASP proteins from focal adhesions, but not the leading edge, has no effect on motility. Constitutive membrane targeting of Ena/VASP proteins inhibits motility. These results are in marked contrast to current models for Ena/VASP function derived mainly from their role in the actin-driven movement of Listeria monocytogenes. Anna Fuller Molecular Oncology Fund Merck & Co., Inc. National Institutes of Health (U.S.) (Grant GM58801) 2014-01-06T16:02:14Z 2014-01-06T16:02:14Z 2000-06 2000-05 Article http://purl.org/eprint/type/JournalArticle 00928674 1097-4172 http://hdl.handle.net/1721.1/83484 Bear, James E, Joseph J Loureiro, Irina Libova, Reinhard Fassler, Jurgen Wehland, and Frank B Gertler. “Negative Regulation of Fibroblast Motility by Ena/VASP Proteins.” Cell 101, no. 7 (June 2000): 717-728. Copyright © 2000 Cell Press https://orcid.org/0000-0003-3214-4554 en_US http://dx.doi.org/10.1016/S0092-8674(00)80884-3 Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier Open Archive |
| spellingShingle | Libova, Irina Bear, James E. Loureiro, Joseph J. Gertler, Frank Fassler, Reinhard Wehland, Jurgen Negative Regulation of Fibroblast Motility by Ena/VASP Proteins |
| title | Negative Regulation of Fibroblast Motility by Ena/VASP Proteins |
| title_full | Negative Regulation of Fibroblast Motility by Ena/VASP Proteins |
| title_fullStr | Negative Regulation of Fibroblast Motility by Ena/VASP Proteins |
| title_full_unstemmed | Negative Regulation of Fibroblast Motility by Ena/VASP Proteins |
| title_short | Negative Regulation of Fibroblast Motility by Ena/VASP Proteins |
| title_sort | negative regulation of fibroblast motility by ena vasp proteins |
| url | http://hdl.handle.net/1721.1/83484 https://orcid.org/0000-0003-3214-4554 |
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