Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing

Transcript leaders (TLs) can have profound effects on mRNA translation and stability. To map TL boundaries genome-wide, we developed TL-sequencing (TL-seq), a technique combining enzymatic capture of m[superscript 7]G-capped mRNA 5′ ends with high-throughput sequencing. TL-seq identified mRNA start...

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Bibliographic Details
Main Authors: Arribere, Joshua Alexander, Gilbert, Wendy
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Cold Spring Harbor Laboratory Press 2014
Online Access:http://hdl.handle.net/1721.1/83927
https://orcid.org/0000-0003-2807-9657