A Polymorphism in npr-1 Is a Behavioral Determinant of Pathogen Susceptibility in C. elegans
The nematode Caenorhabditis elegans responds to pathogenic bacteria with conserved innate immune responses and pathogen avoidance behaviors. We investigated natural variation in C. elegans resistance to pathogen infection. With the use of quantitative genetic analysis, we determined that the pathoge...
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| Format: | Article |
| Language: | en_US |
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American Association for the Advancement of Science (AAAS)
2014
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| Online Access: | http://hdl.handle.net/1721.1/84622 https://orcid.org/0000-0002-4109-5152 |
| _version_ | 1826201214918852608 |
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| author | Reddy, Kirthi C. Kim, Dennis H. Andersen, Erik C. Kruglyak, Leonid |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Reddy, Kirthi C. Kim, Dennis H. Andersen, Erik C. Kruglyak, Leonid |
| author_sort | Reddy, Kirthi C. |
| collection | MIT |
| description | The nematode Caenorhabditis elegans responds to pathogenic bacteria with conserved innate immune responses and pathogen avoidance behaviors. We investigated natural variation in C. elegans resistance to pathogen infection. With the use of quantitative genetic analysis, we determined that the pathogen susceptibility difference between the laboratory wild-type strain N2 and the wild isolate CB4856 is caused by a polymorphism in the npr-1 gene, which encodes a homolog of the mammalian neuropeptide Y receptor. We show that the mechanism of NPR-1–mediated pathogen resistance is through oxygen-dependent behavioral avoidance rather than direct regulation of innate immunity. For C. elegans, bacteria represent food but also a potential source of infection. Our data underscore the importance of behavioral responses to oxygen levels in finding an optimal balance between these potentially conflicting cues. |
| first_indexed | 2024-09-23T11:48:20Z |
| format | Article |
| id | mit-1721.1/84622 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:48:20Z |
| publishDate | 2014 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/846222022-09-27T22:01:05Z A Polymorphism in npr-1 Is a Behavioral Determinant of Pathogen Susceptibility in C. elegans Reddy, Kirthi C. Kim, Dennis H. Andersen, Erik C. Kruglyak, Leonid Massachusetts Institute of Technology. Department of Biology Reddy, Kirthi C. Kim, Dennis H. The nematode Caenorhabditis elegans responds to pathogenic bacteria with conserved innate immune responses and pathogen avoidance behaviors. We investigated natural variation in C. elegans resistance to pathogen infection. With the use of quantitative genetic analysis, we determined that the pathogen susceptibility difference between the laboratory wild-type strain N2 and the wild isolate CB4856 is caused by a polymorphism in the npr-1 gene, which encodes a homolog of the mammalian neuropeptide Y receptor. We show that the mechanism of NPR-1–mediated pathogen resistance is through oxygen-dependent behavioral avoidance rather than direct regulation of innate immunity. For C. elegans, bacteria represent food but also a potential source of infection. Our data underscore the importance of behavioral responses to oxygen levels in finding an optimal balance between these potentially conflicting cues. National Institutes of Health (U.S.) (Grant GM084477) Burroughs Wellcome Fund Jane Coffin Childs Memorial Fund for Medical Research 2014-01-31T17:19:01Z 2014-01-31T17:19:01Z 2009-01 2008-09 Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 http://hdl.handle.net/1721.1/84622 Reddy, K. C. et al. “A Polymorphism in Npr-1 Is a Behavioral Determinant of Pathogen Susceptibility in C. Elegans.” Science 323.5912 (2009): 382–384. https://orcid.org/0000-0002-4109-5152 en_US http://dx.doi.org/10.1126/science.1166527 Science Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Reddy, Kirthi C. Kim, Dennis H. Andersen, Erik C. Kruglyak, Leonid A Polymorphism in npr-1 Is a Behavioral Determinant of Pathogen Susceptibility in C. elegans |
| title | A Polymorphism in npr-1 Is a Behavioral Determinant of Pathogen Susceptibility in C. elegans |
| title_full | A Polymorphism in npr-1 Is a Behavioral Determinant of Pathogen Susceptibility in C. elegans |
| title_fullStr | A Polymorphism in npr-1 Is a Behavioral Determinant of Pathogen Susceptibility in C. elegans |
| title_full_unstemmed | A Polymorphism in npr-1 Is a Behavioral Determinant of Pathogen Susceptibility in C. elegans |
| title_short | A Polymorphism in npr-1 Is a Behavioral Determinant of Pathogen Susceptibility in C. elegans |
| title_sort | polymorphism in npr 1 is a behavioral determinant of pathogen susceptibility in c elegans |
| url | http://hdl.handle.net/1721.1/84622 https://orcid.org/0000-0002-4109-5152 |
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