Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells
Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Here...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/85010 https://orcid.org/0000-0003-0396-2443 https://orcid.org/0000-0001-5629-4798 |
| _version_ | 1811081970070847488 |
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| author | Lee, Samuel Ma, Minglin Kim, Soo Min Guye, Patrick Pancoast, James R. Weiss, Ron Lee, Richard T. Anderson, Daniel Griffith Hammond, Paula T Li, W. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Lee, Samuel Ma, Minglin Kim, Soo Min Guye, Patrick Pancoast, James R. Weiss, Ron Lee, Richard T. Anderson, Daniel Griffith Hammond, Paula T Li, W. |
| author_sort | Lee, Samuel |
| collection | MIT |
| description | Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-vivo cell microenvironment by culturing primary β-cells in direct contact with ‘synthetic neighbors', cell-sized soft polymer microbeads that were modified with cell-cell signaling factors as well as components from pancreatic-tissue-specific ECMs. This biomimetic 3D microenvironment was able to promote native cell-cell and cell-ECM interactions. We obtained sustained maintenance of β-cell function in vitro enhanced cell viability from the few days usually observed in 2D culture to periods exceeding three weeks, with enhanced β-cell stability and insulin production. Our approach can be extended to create a general 3D culture platform for other cell types. |
| first_indexed | 2024-09-23T11:55:18Z |
| format | Article |
| id | mit-1721.1/85010 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:55:18Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/850102022-09-27T22:51:16Z Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells Lee, Samuel Ma, Minglin Kim, Soo Min Guye, Patrick Pancoast, James R. Weiss, Ron Lee, Richard T. Anderson, Daniel Griffith Hammond, Paula T Li, W. Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Li, Wei Ma, Minglin Anderson, Daniel Griffith Hammond, Paula T. Guye, Patrick Weiss, Ron Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-vivo cell microenvironment by culturing primary β-cells in direct contact with ‘synthetic neighbors', cell-sized soft polymer microbeads that were modified with cell-cell signaling factors as well as components from pancreatic-tissue-specific ECMs. This biomimetic 3D microenvironment was able to promote native cell-cell and cell-ECM interactions. We obtained sustained maintenance of β-cell function in vitro enhanced cell viability from the few days usually observed in 2D culture to periods exceeding three weeks, with enhanced β-cell stability and insulin production. Our approach can be extended to create a general 3D culture platform for other cell types. National Science Foundation (U.S.). Emergent Behaviors of Integrated Cellular Systems David H. Koch Institute for Integrative Cancer Research at MIT Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies 2014-02-19T17:30:39Z 2014-02-19T17:30:39Z 2013-10 2013-08 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/85010 Li, Wei, Samuel Lee, Minglin Ma, Soo Min Kim, Patrick Guye, James R. Pancoast, Daniel G. Anderson, Ron Weiss, Richard T. Lee, and Paula T. Hammond. “Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells.” Scientific Reports 3 (October 4, 2013). https://orcid.org/0000-0003-0396-2443 https://orcid.org/0000-0001-5629-4798 en_US http://dx.doi.org/10.1038/srep02863 Scientific Reports http://creativecommons.org/licenses/by-nc-nd/3.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Lee, Samuel Ma, Minglin Kim, Soo Min Guye, Patrick Pancoast, James R. Weiss, Ron Lee, Richard T. Anderson, Daniel Griffith Hammond, Paula T Li, W. Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
| title | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
| title_full | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
| title_fullStr | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
| title_full_unstemmed | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
| title_short | Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells |
| title_sort | microbead based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β cells |
| url | http://hdl.handle.net/1721.1/85010 https://orcid.org/0000-0003-0396-2443 https://orcid.org/0000-0001-5629-4798 |
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