Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry
Chimeric proteins, including bispecific antibodies, are biological tools with therapeutic applications. Genetic fusion and ligation methods allow the creation of N-to-C and C-to-N fused recombinant proteins, but not unnaturally linked N-to-N and C-to-C fusion proteins. This protocol describes a simp...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/85585 https://orcid.org/0000-0002-1090-6071 |