Site-specific N-terminal labeling of proteins using sortase-mediated reactions

Site-specific N-terminal labeling of proteins using sortase-mediated reactions

This protocol describes the use of sortase-mediated reactions to label the N terminus of any given protein of interest. The sortase recognition sequence, LPXTG (for Staphylococcus aureus sortase A) or LPXTA (for Streptococcus pyogenes sortase A), can be appended to a variety of probes such as fluoro...

Full description

Bibliographic Details
Main Authors: Theile, Christopher S., Witte, Martin D., Blom, Annet E. M., Kundrat, Lenka, Guimaraes, Carla P., Ploegh, Hidde
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Nature Publishing Group 2014
Online Access:http://hdl.handle.net/1721.1/85593
https://orcid.org/0000-0002-1090-6071

Internet

http://hdl.handle.net/1721.1/85593
https://orcid.org/0000-0002-1090-6071

Similar Items