An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum
Background: The construction of plasmid vectors for transgene expression in the malaria parasite, Plasmodium falciparum, presents major technical hurdles. Traditional molecular cloning by restriction and ligation often yields deletions and re-arrangements when assembling low-complexity (A + T)-rich...
Main Authors: | , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
BioMed Central Ltd
2014
|
Online Access: | http://hdl.handle.net/1721.1/86003 https://orcid.org/0000-0002-7779-2216 https://orcid.org/0000-0002-6250-8796 |