An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum

Background: The construction of plasmid vectors for transgene expression in the malaria parasite, Plasmodium falciparum, presents major technical hurdles. Traditional molecular cloning by restriction and ligation often yields deletions and re-arrangements when assembling low-complexity (A + T)-rich...

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Bibliographic Details
Main Authors: Lee, Marcus CS, Fidock, David A, Wagner, Jeffrey Charles, Goldfless, Stephen Jacob, Maddur Ganesan, Suresh, Niles, Jacquin
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:English
Published: BioMed Central Ltd 2014
Online Access:http://hdl.handle.net/1721.1/86003
https://orcid.org/0000-0002-7779-2216
https://orcid.org/0000-0002-6250-8796