High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome
Chromosomes must be highly compacted and organized within cells, but how this is achieved in vivo remains poorly understood. We report the use of chromosome conformation capture coupled with deep sequencing (Hi-C) to map the structure of bacterial chromosomes. Analysis of Hi-C data and polymer model...
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| Format: | Article |
| Language: | en_US |
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American Association for the Advancement of Science (AAAS)
2014
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| Online Access: | http://hdl.handle.net/1721.1/86053 https://orcid.org/0000-0003-4764-8851 https://orcid.org/0000-0002-0785-5410 https://orcid.org/0000-0002-8288-7607 https://orcid.org/0000-0002-5320-2728 |
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| author | Imakaev, Maksim Viktorovich Le, Tung Mirny, Leonid A Laub, Michael T |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Imakaev, Maksim Viktorovich Le, Tung Mirny, Leonid A Laub, Michael T |
| author_sort | Imakaev, Maksim Viktorovich |
| collection | MIT |
| description | Chromosomes must be highly compacted and organized within cells, but how this is achieved in vivo remains poorly understood. We report the use of chromosome conformation capture coupled with deep sequencing (Hi-C) to map the structure of bacterial chromosomes. Analysis of Hi-C data and polymer modeling indicates that the Caulobacter crescentus chromosome consists of multiple, largely independent spatial domains that are probably composed of supercoiled plectonemes arrayed into a bottle brush–like fiber. These domains are stable throughout the cell cycle and are reestablished concomitantly with DNA replication. We provide evidence that domain boundaries are established by highly expressed genes and the formation of plectoneme-free regions, whereas the histone-like protein HU and SMC (structural maintenance of chromosomes) promote short-range compaction and the colinearity of chromosomal arms, respectively. Collectively, our results reveal general principles for the organization and structure of chromosomes in vivo. |
| first_indexed | 2024-09-23T10:16:09Z |
| format | Article |
| id | mit-1721.1/86053 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:16:09Z |
| publishDate | 2014 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/860532022-09-30T20:00:06Z High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome Imakaev, Maksim Viktorovich Le, Tung Mirny, Leonid A Laub, Michael T Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Physics Massachusetts Institute of Technology. School of Engineering Laub, Michael T. Le, Tung Ba Khanh Imakaev, Maksim Viktorovich Mirny, Leonid A. Laub, Michael T. Chromosomes must be highly compacted and organized within cells, but how this is achieved in vivo remains poorly understood. We report the use of chromosome conformation capture coupled with deep sequencing (Hi-C) to map the structure of bacterial chromosomes. Analysis of Hi-C data and polymer modeling indicates that the Caulobacter crescentus chromosome consists of multiple, largely independent spatial domains that are probably composed of supercoiled plectonemes arrayed into a bottle brush–like fiber. These domains are stable throughout the cell cycle and are reestablished concomitantly with DNA replication. We provide evidence that domain boundaries are established by highly expressed genes and the formation of plectoneme-free regions, whereas the histone-like protein HU and SMC (structural maintenance of chromosomes) promote short-range compaction and the colinearity of chromosomal arms, respectively. Collectively, our results reveal general principles for the organization and structure of chromosomes in vivo. National Institutes of Health (U.S.) (Grant R01GM082899) National Institutes of Health (U.S.) (Grant U54CA143874) 2014-04-07T15:00:12Z 2014-04-07T15:00:12Z 2013-10 2013-06 Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 http://hdl.handle.net/1721.1/86053 Le, T. B. K., M. V. Imakaev, L. A. Mirny, and M. T. Laub. “High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome.” Science 342, no. 6159 (November 8, 2013): 731–734. https://orcid.org/0000-0003-4764-8851 https://orcid.org/0000-0002-0785-5410 https://orcid.org/0000-0002-8288-7607 https://orcid.org/0000-0002-5320-2728 en_US http://dx.doi.org/10.1126/science.1242059 Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Association for the Advancement of Science (AAAS) Laub via Courtney Crummett |
| spellingShingle | Imakaev, Maksim Viktorovich Le, Tung Mirny, Leonid A Laub, Michael T High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome |
| title | High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome |
| title_full | High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome |
| title_fullStr | High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome |
| title_full_unstemmed | High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome |
| title_short | High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome |
| title_sort | high resolution mapping of the spatial organization of a bacterial chromosome |
| url | http://hdl.handle.net/1721.1/86053 https://orcid.org/0000-0003-4764-8851 https://orcid.org/0000-0002-0785-5410 https://orcid.org/0000-0002-8288-7607 https://orcid.org/0000-0002-5320-2728 |
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