Laser-Assisted In Vitro Fertilization Facilitates Fertilization of Vitrified-Warmed C57BL/6 Mouse Oocytes with Fresh and Frozen-Thawed Spermatozoa, Producing Live Pups

Laser-Assisted In Vitro Fertilization Facilitates Fertilization of Vitrified-Warmed C57BL/6 Mouse Oocytes with Fresh and Frozen-Thawed Spermatozoa, Producing Live Pups

The utility of cryopreserved mouse gametes for reproduction of transgenic mice depends on development of assisted reproductive technologies, including vitrification of unfertilized mouse oocytes. Due to hardening of the zona pellucida, spermatozoa are often unable to penetrate vitrified-warmed (V-W)...

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Bibliographic Details
Main Authors: Qi, Peimin, Rosalia, Elizabeth K., Discua, Allan R., Mkandawire, John M., Garcia, Alexis, Woods, Stephanie, Chavarria, Tony E, Fox, James G
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Public Library of Science 2014
Online Access:http://hdl.handle.net/1721.1/86081
https://orcid.org/0000-0001-9307-6116

Internet

http://hdl.handle.net/1721.1/86081
https://orcid.org/0000-0001-9307-6116

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