High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons
The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/86091 https://orcid.org/0000-0003-2673-1672 |
| _version_ | 1826211796304789504 |
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| author | Logan, David J. Saulnier, Jessica Lam, Daniel Johnson, Caroline Root, David E. Carpenter, Anne E. Sabatini, Bernardo L. Nieland, Thomas J |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Logan, David J. Saulnier, Jessica Lam, Daniel Johnson, Caroline Root, David E. Carpenter, Anne E. Sabatini, Bernardo L. Nieland, Thomas J |
| author_sort | Logan, David J. |
| collection | MIT |
| description | The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such as Alzheimer's disease, intellectual disability, and autism spectrum disorders. Many genes controlling synaptogenesis have been identified, but lack of facile experimental systems has made systematic discovery of regulators of synaptogenesis challenging. Thus, we created a high-throughput platform to study excitatory and inhibitory synapse development in primary neuronal cultures and used a lentiviral RNA interference library to identify novel regulators of synapse formation. This methodology is broadly applicable for high-throughput screening of genes and drugs that may rescue or improve synaptic dysfunction associated with cognitive function and neurological disorders. |
| first_indexed | 2024-09-23T15:11:32Z |
| format | Article |
| id | mit-1721.1/86091 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:11:32Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/860912022-09-29T13:16:22Z High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons Logan, David J. Saulnier, Jessica Lam, Daniel Johnson, Caroline Root, David E. Carpenter, Anne E. Sabatini, Bernardo L. Nieland, Thomas J Massachusetts Institute of Technology. Department of Biology Nieland, Thomas J. The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such as Alzheimer's disease, intellectual disability, and autism spectrum disorders. Many genes controlling synaptogenesis have been identified, but lack of facile experimental systems has made systematic discovery of regulators of synaptogenesis challenging. Thus, we created a high-throughput platform to study excitatory and inhibitory synapse development in primary neuronal cultures and used a lentiviral RNA interference library to identify novel regulators of synapse formation. This methodology is broadly applicable for high-throughput screening of genes and drugs that may rescue or improve synaptic dysfunction associated with cognitive function and neurological disorders. National Institutes of Health (U.S.) (MH095096) National Institutes of Health (U.S.) (R01 GM089652) 2014-04-09T20:47:38Z 2014-04-09T20:47:38Z 2014-03 2013-11 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/86091 Nieland, Thomas J. F., David J. Logan, Jessica Saulnier, Daniel Lam, Caroline Johnson, David E. Root, Anne E. Carpenter, and Bernardo L. Sabatini. “High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons.” Edited by Lin Mei. PLoS ONE 9, no. 3 (March 14, 2014): e91744. https://orcid.org/0000-0003-2673-1672 en_US http://dx.doi.org/10.1371/journal.pone.0091744 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science PLoS |
| spellingShingle | Logan, David J. Saulnier, Jessica Lam, Daniel Johnson, Caroline Root, David E. Carpenter, Anne E. Sabatini, Bernardo L. Nieland, Thomas J High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons |
| title | High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons |
| title_full | High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons |
| title_fullStr | High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons |
| title_full_unstemmed | High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons |
| title_short | High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons |
| title_sort | high content image analysis identifies novel regulators of synaptogenesis in a high throughput rnai screen of primary neurons |
| url | http://hdl.handle.net/1721.1/86091 https://orcid.org/0000-0003-2673-1672 |
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