Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
Fluorescence time-lapse microscopy has become a powerful tool in the study of many biological processes at the single-cell level. In particular, movies depicting the temporal dependence of gene expression provide insight into the dynamics of its regulation; however, there are many technical challeng...
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Format: | Article |
Language: | en_US |
Published: |
MyJoVE Corporation
2014
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Online Access: | http://hdl.handle.net/1721.1/89640 |