Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Ideal cell-free expression systems can theoretically emulate an in vivo cellular environment in a controlled in vitro platform. This is useful for expressing proteins and genetic circuits in a controlled manner as well as for providing a prototyping environment for synthetic biology. To achieve the...

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Bibliographic Details
Main Authors: Sun, Zachary Z., Hayes, Clarmyra A., Shin, Jonghyeon, Caschera, Filippo, Murray, Richard M., Noireaux, Vincent
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: MyJoVE Corporation 2014
Online Access:http://hdl.handle.net/1721.1/89658
https://orcid.org/0000-0002-6792-7092

Internet

http://hdl.handle.net/1721.1/89658
https://orcid.org/0000-0002-6792-7092

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