Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA delivery
The safe, targeted and effective delivery of gene therapeutics remains a significant barrier to their broad clinical application. Here we develop a magnetic nucleic acid delivery system composed of iron oxide nanoparticles and cationic lipid-like materials termed lipidoids. Coated nanoparticles are...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2014
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| Online Access: | http://hdl.handle.net/1721.1/91255 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
| _version_ | 1811088110644101120 |
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| author | Jiang, Shan Eltoukhy, Ahmed A. Anderson, Daniel Griffith Love, Kevin T Langer, Robert S |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Jiang, Shan Eltoukhy, Ahmed A. Anderson, Daniel Griffith Love, Kevin T Langer, Robert S |
| author_sort | Jiang, Shan |
| collection | MIT |
| description | The safe, targeted and effective delivery of gene therapeutics remains a significant barrier to their broad clinical application. Here we develop a magnetic nucleic acid delivery system composed of iron oxide nanoparticles and cationic lipid-like materials termed lipidoids. Coated nanoparticles are capable of delivering DNA and siRNA to cells in culture. The mean hydrodynamic size of these nanoparticles was systematically varied and optimized for delivery. While nanoparticles of different sizes showed similar siRNA delivery efficiency, nanoparticles of 50–100 nm displayed optimal DNA delivery activity. The application of an external magnetic field significantly enhanced the efficiency of nucleic acid delivery, with performance exceeding that of the commercially available lipid-based reagent, Lipofectamine 2000. The iron oxide nanoparticle delivery platform developed here offers the potential for magnetically guided targeting, as well as an opportunity to combine gene therapy with MRI imaging and magnetic hyperthermia. |
| first_indexed | 2024-09-23T13:56:26Z |
| format | Article |
| id | mit-1721.1/91255 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:56:26Z |
| publishDate | 2014 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/912552022-09-28T17:13:33Z Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA delivery Jiang, Shan Eltoukhy, Ahmed A. Anderson, Daniel Griffith Love, Kevin T Langer, Robert S Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Jiang, Shan Eltoukhy, Ahmed A. Love, Kevin T. Langer, Robert Anderson, Daniel Griffith The safe, targeted and effective delivery of gene therapeutics remains a significant barrier to their broad clinical application. Here we develop a magnetic nucleic acid delivery system composed of iron oxide nanoparticles and cationic lipid-like materials termed lipidoids. Coated nanoparticles are capable of delivering DNA and siRNA to cells in culture. The mean hydrodynamic size of these nanoparticles was systematically varied and optimized for delivery. While nanoparticles of different sizes showed similar siRNA delivery efficiency, nanoparticles of 50–100 nm displayed optimal DNA delivery activity. The application of an external magnetic field significantly enhanced the efficiency of nucleic acid delivery, with performance exceeding that of the commercially available lipid-based reagent, Lipofectamine 2000. The iron oxide nanoparticle delivery platform developed here offers the potential for magnetically guided targeting, as well as an opportunity to combine gene therapy with MRI imaging and magnetic hyperthermia. National Heart, Lung, and Blood Institute National Institutes of Health (U.S.) (Program of Excellence in Nanotechnology (PEN) Award, Contract #HHSN268201000045C) 2014-10-31T17:54:46Z 2014-10-31T17:54:46Z 2013-03 2013-01 Article http://purl.org/eprint/type/JournalArticle 1530-6984 1530-6992 http://hdl.handle.net/1721.1/91255 Jiang, Shan, Ahmed A. Eltoukhy, Kevin T. Love, Robert Langer, and Daniel G. Anderson. “Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA Delivery.” Nano Lett. 13, no. 3 (March 13, 2013): 1059–1064. https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1021/nl304287a Nano Letters Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Jiang, Shan Eltoukhy, Ahmed A. Anderson, Daniel Griffith Love, Kevin T Langer, Robert S Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA delivery |
| title | Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA delivery |
| title_full | Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA delivery |
| title_fullStr | Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA delivery |
| title_full_unstemmed | Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA delivery |
| title_short | Lipidoid-Coated Iron Oxide Nanoparticles for Efficient DNA and siRNA delivery |
| title_sort | lipidoid coated iron oxide nanoparticles for efficient dna and sirna delivery |
| url | http://hdl.handle.net/1721.1/91255 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
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