Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers
Anionic, monolayer-protected gold nanoparticles (AuNPs) have been shown to nondisruptively penetrate cellular membranes. Here, we show that a critical first step in the penetration process is potentially the fusion of such AuNPs with lipid bilayers. Free energy calculations, experiments on unilamell...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2014
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| Online Access: | http://hdl.handle.net/1721.1/91636 https://orcid.org/0000-0001-5554-1283 |
| _version_ | 1826212905183346688 |
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| author | Van Lehn, Reid C. Atukorale, Prabhani U. Carney, Randy P. Yang, Yu-Sang Sabrina Stellacci, Francesco Irvine, Darrell J. Alexander-Katz, Alfredo |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Van Lehn, Reid C. Atukorale, Prabhani U. Carney, Randy P. Yang, Yu-Sang Sabrina Stellacci, Francesco Irvine, Darrell J. Alexander-Katz, Alfredo |
| author_sort | Van Lehn, Reid C. |
| collection | MIT |
| description | Anionic, monolayer-protected gold nanoparticles (AuNPs) have been shown to nondisruptively penetrate cellular membranes. Here, we show that a critical first step in the penetration process is potentially the fusion of such AuNPs with lipid bilayers. Free energy calculations, experiments on unilamellar and multilamellar vesicles, and cell studies all support this hypothesis. Furthermore, we show that fusion is only favorable for AuNPs with core diameters below a critical size that depends on the monolayer composition. |
| first_indexed | 2024-09-23T15:40:00Z |
| format | Article |
| id | mit-1721.1/91636 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:40:00Z |
| publishDate | 2014 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/916362022-09-29T15:20:20Z Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers Van Lehn, Reid C. Atukorale, Prabhani U. Carney, Randy P. Yang, Yu-Sang Sabrina Stellacci, Francesco Irvine, Darrell J. Alexander-Katz, Alfredo Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Koch Institute for Integrative Cancer Research at MIT Van Lehn, Reid C. Yang, Yu-Sang Sabrina Irvine, Darrell J. Alexander-Katz, Alfredo Atukorale, Prabhani U. Anionic, monolayer-protected gold nanoparticles (AuNPs) have been shown to nondisruptively penetrate cellular membranes. Here, we show that a critical first step in the penetration process is potentially the fusion of such AuNPs with lipid bilayers. Free energy calculations, experiments on unilamellar and multilamellar vesicles, and cell studies all support this hypothesis. Furthermore, we show that fusion is only favorable for AuNPs with core diameters below a critical size that depends on the monolayer composition. National Science Foundation (U.S.). Graduate Research Fellowship Program National Science Foundation (U.S.). Materials Research Science and Engineering Centers (Program) (Award DMR-0819762) National Cancer Institute (U.S.) (Award U54CA143874) United States. Army Research Office (Contract W911NF-13-D-0001) United States. Army Research Office (Contract W911NF-07-D-0004, T.O. 8) 2014-11-20T14:24:52Z 2014-11-20T14:24:52Z 2013-08 2013-07 Article http://purl.org/eprint/type/JournalArticle 1530-6984 1530-6992 http://hdl.handle.net/1721.1/91636 Van Lehn, Reid C., Prabhani U. Atukorale, Randy P. Carney, Yu-Sang Yang, Francesco Stellacci, Darrell J. Irvine, and Alfredo Alexander-Katz. “Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers.” Nano Lett. 13, no. 9 (September 11, 2013): 4060–4067. https://orcid.org/0000-0001-5554-1283 en_US http://dx.doi.org/10.1021/nl401365n Nano Letters Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Van Lehn, Reid C. Atukorale, Prabhani U. Carney, Randy P. Yang, Yu-Sang Sabrina Stellacci, Francesco Irvine, Darrell J. Alexander-Katz, Alfredo Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers |
| title | Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers |
| title_full | Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers |
| title_fullStr | Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers |
| title_full_unstemmed | Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers |
| title_short | Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers |
| title_sort | effect of particle diameter and surface composition on the spontaneous fusion of monolayer protected gold nanoparticles with lipid bilayers |
| url | http://hdl.handle.net/1721.1/91636 https://orcid.org/0000-0001-5554-1283 |
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