Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls
Decades ago, two nonoverlapping crypt stem cell populations were proposed: Leblond’s Crypt Base Columnar (CBC) cell and Potten’s +4 cell. The identification of CBC markers including Lgr5 has confirmed Leblond’s predictions that CBC cells are anatomically distinct, long-lived stem cells that permanen...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2014
|
| Online Access: | http://hdl.handle.net/1721.1/91960 |
| _version_ | 1826188901314723840 |
|---|---|
| author | Barker, Nick van Oudenaarden, Alexander Clevers, Hans van Oudenaarden, Alexander |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Barker, Nick van Oudenaarden, Alexander Clevers, Hans van Oudenaarden, Alexander |
| author_sort | Barker, Nick |
| collection | MIT |
| description | Decades ago, two nonoverlapping crypt stem cell populations were proposed: Leblond’s Crypt Base Columnar (CBC) cell and Potten’s +4 cell. The identification of CBC markers including Lgr5 has confirmed Leblond’s predictions that CBC cells are anatomically distinct, long-lived stem cells that permanently cycle. While Potten originally described +4 cells as proliferative and unusually radiation-sensitive, recent efforts to identify +4 stem cells have focused on the identification of cells that are quiescent and radiation-resistant. Here, we describe commonalities and discrepancies between the individual studies and discuss challenges of marker-based lineage tracing. |
| first_indexed | 2024-09-23T08:06:46Z |
| format | Article |
| id | mit-1721.1/91960 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:06:46Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/919602022-09-23T10:58:15Z Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls Barker, Nick van Oudenaarden, Alexander Clevers, Hans van Oudenaarden, Alexander Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Physics van Oudenaarden, Alexander Decades ago, two nonoverlapping crypt stem cell populations were proposed: Leblond’s Crypt Base Columnar (CBC) cell and Potten’s +4 cell. The identification of CBC markers including Lgr5 has confirmed Leblond’s predictions that CBC cells are anatomically distinct, long-lived stem cells that permanently cycle. While Potten originally described +4 cells as proliferative and unusually radiation-sensitive, recent efforts to identify +4 stem cells have focused on the identification of cells that are quiescent and radiation-resistant. Here, we describe commonalities and discrepancies between the individual studies and discuss challenges of marker-based lineage tracing. 2014-12-01T17:17:48Z 2014-12-01T17:17:48Z 2012-10 Article http://purl.org/eprint/type/JournalArticle 19345909 http://hdl.handle.net/1721.1/91960 Barker, Nick, Alexander van Oudenaarden, and Hans Clevers. “Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls.” Cell Stem Cell 11, no. 4 (October 2012): 452–460. © 2012 Elsevier Inc. en_US http://dx.doi.org/10.1016/j.stem.2012.09.009 Cell Stem Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier |
| spellingShingle | Barker, Nick van Oudenaarden, Alexander Clevers, Hans van Oudenaarden, Alexander Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls |
| title | Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls |
| title_full | Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls |
| title_fullStr | Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls |
| title_full_unstemmed | Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls |
| title_short | Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls |
| title_sort | identifying the stem cell of the intestinal crypt strategies and pitfalls |
| url | http://hdl.handle.net/1721.1/91960 |
| work_keys_str_mv | AT barkernick identifyingthestemcelloftheintestinalcryptstrategiesandpitfalls AT vanoudenaardenalexander identifyingthestemcelloftheintestinalcryptstrategiesandpitfalls AT clevershans identifyingthestemcelloftheintestinalcryptstrategiesandpitfalls AT vanoudenaardenalexander identifyingthestemcelloftheintestinalcryptstrategiesandpitfalls |