Mycobacterial RNA isolation optimized for non-coding RNA: high fidelity isolation of 5S rRNA from Mycobacterium bovis BCG reveals novel post-transcriptional processing and a complete spectrum of modified ribonucleosides

Mycobacterial RNA isolation optimized for non-coding RNA: high fidelity isolation of 5S rRNA from Mycobacterium bovis BCG reveals novel post-transcriptional processing and a complete spectrum of modified ribonucleosides

A major challenge in the study of mycobacterial RNA biology is the lack of a comprehensive RNA isolation method that overcomes the unusual cell wall to faithfully yield the full spectrum of non-coding RNA (ncRNA) species. Here, we describe a simple and robust procedure optimized for the isolation of...

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Bibliographic Details
Main Authors: Hia, Fabian, Chionh, Yok Hian, Pang, Yan Ling Joy, DeMott, Michael S., McBee, Megan E., Dedon, Peter C.
Other Authors: Massachusetts Institute of Technology. Center for Environmental Health Sciences
Format: Article
Language:en_US
Published: Oxford University Press 2015
Online Access:http://hdl.handle.net/1721.1/94515
https://orcid.org/0000-0003-2673-5606
https://orcid.org/0000-0003-0011-3067
https://orcid.org/0000-0001-7380-4075

Internet

http://hdl.handle.net/1721.1/94515
https://orcid.org/0000-0003-2673-5606
https://orcid.org/0000-0003-0011-3067
https://orcid.org/0000-0001-7380-4075

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