Noninvasive optical inhibition with a red-shifted microbial rhodopsin
Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) sali...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2015
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| Online Access: | http://hdl.handle.net/1721.1/95967 https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0001-6754-0333 https://orcid.org/0000-0002-7002-1275 https://orcid.org/0000-0002-5680-2630 https://orcid.org/0000-0002-5534-6020 https://orcid.org/0000-0001-9969-4281 https://orcid.org/0000-0002-0419-3351 |
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| author | Miri, Mitra L. Busskamp, Volker Young, Andrew Ogawa, Masaaki Ramanlal, Shreshtha B. Forest, Craig R. Chow, Brian Y. Han, Xue Lin, Yingxi Roska, Botond Cardin, Jessica A. Chuong, Amy S. Acker, Leah Christine Henninger, Michael Alan Kodandaramaiah, Suhasa Bangalo Bandler, Rachel C. Allen, Brian Douglas Boyden, Edward Stuart Sorensen, Andreas Toft Klapoetke, Nathan Cao Matthews, Gillian A. Tye, Kay |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Miri, Mitra L. Busskamp, Volker Young, Andrew Ogawa, Masaaki Ramanlal, Shreshtha B. Forest, Craig R. Chow, Brian Y. Han, Xue Lin, Yingxi Roska, Botond Cardin, Jessica A. Chuong, Amy S. Acker, Leah Christine Henninger, Michael Alan Kodandaramaiah, Suhasa Bangalo Bandler, Rachel C. Allen, Brian Douglas Boyden, Edward Stuart Sorensen, Andreas Toft Klapoetke, Nathan Cao Matthews, Gillian A. Tye, Kay |
| author_sort | Miri, Mitra L. |
| collection | MIT |
| description | Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light–induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience. |
| first_indexed | 2024-09-23T12:18:50Z |
| format | Article |
| id | mit-1721.1/95967 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:18:50Z |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/959672022-09-28T00:58:01Z Noninvasive optical inhibition with a red-shifted microbial rhodopsin Miri, Mitra L. Busskamp, Volker Young, Andrew Ogawa, Masaaki Ramanlal, Shreshtha B. Forest, Craig R. Chow, Brian Y. Han, Xue Lin, Yingxi Roska, Botond Cardin, Jessica A. Chuong, Amy S. Acker, Leah Christine Henninger, Michael Alan Kodandaramaiah, Suhasa Bangalo Bandler, Rachel C. Allen, Brian Douglas Boyden, Edward Stuart Sorensen, Andreas Toft Klapoetke, Nathan Cao Matthews, Gillian A. Tye, Kay Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Physics McGovern Institute for Brain Research at MIT Picower Institute for Learning and Memory Program in Media Arts and Sciences (Massachusetts Institute of Technology) Chuong, Amy S. Acker, Leah Christine Henninger, Michael Alan Kodandaramaiah, Suhasa Bangalo Ogawa, Masaaki Bandler, Rachel C. Allen, Brian Douglas Boyden, Edward Stuart Sorensen, Andreas Toft Young, Andrew Klapoetke, Nathan Cao Lin, Yingxi Matthews, Gillian A. Tye, Kay Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light–induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience. McGovern Institute for Brain Research at MIT (Razin Fellowship) United States. Defense Advanced Research Projects Agency. Living Foundries Program (HR0011-12-C-0068) Harvard-MIT Joint Research Grants Program in Basic Neuroscience Human Frontier Science Program (Strasbourg, France) Institution of Engineering and Technology (A. F. Harvey Prize) McGovern Institute for Brain Research at MIT. Neurotechnology (MINT) Program New York Stem Cell Foundation (Robertson Investigator Award) National Institutes of Health (U.S.) (New Innovator Award 1DP2OD002002) National Institute of General Medical Sciences (U.S.) (EUREKA Award 1R01NS075421) National Institutes of Health (U.S.) (Grant 1R01DA029639) National Institutes of Health (U.S.) (Grant 1RC1MH088182) National Institutes of Health (U.S.) (Grant 1R01NS067199) National Science Foundation (U.S.) (Career Award CBET 1053233) National Science Foundation (U.S.) (Grant EFRI0835878) National Science Foundation (U.S.) (Grant DMS0848804) Society for Neuroscience (Research Award for Innovation in Neuroscience) Wallace H. Coulter Foundation National Institutes of Health (U.S.) (RO1 MH091220-01) Whitehall Foundation Esther A. & Joseph Klingenstein Fund, Inc. JPB Foundation PIIF Funding National Institute of Mental Health (U.S.) (R01-MH102441-01) National Institutes of Health (U.S.) (DP2-OD-017366-01) Massachusetts Institute of Technology. Simons Center for the Social Brain 2015-03-11T20:42:25Z 2015-03-11T20:42:25Z 2014-07 2014-02 Article http://purl.org/eprint/type/JournalArticle 1097-6256 1546-1726 http://hdl.handle.net/1721.1/95967 Chuong, Amy S, Mitra L Miri, Volker Busskamp, Gillian A C Matthews, Leah C Acker, Andreas T Sorensen, Andrew Young, et al. “Noninvasive Optical Inhibition with a Red-Shifted Microbial Rhodopsin.” Nat Neurosci 17, no. 8 (July 6, 2014): 1123–1129. https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0001-6754-0333 https://orcid.org/0000-0002-7002-1275 https://orcid.org/0000-0002-5680-2630 https://orcid.org/0000-0002-5534-6020 https://orcid.org/0000-0001-9969-4281 https://orcid.org/0000-0002-0419-3351 en_US http://dx.doi.org/10.1038/nn.3752 Nature Neuroscience Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Miri, Mitra L. Busskamp, Volker Young, Andrew Ogawa, Masaaki Ramanlal, Shreshtha B. Forest, Craig R. Chow, Brian Y. Han, Xue Lin, Yingxi Roska, Botond Cardin, Jessica A. Chuong, Amy S. Acker, Leah Christine Henninger, Michael Alan Kodandaramaiah, Suhasa Bangalo Bandler, Rachel C. Allen, Brian Douglas Boyden, Edward Stuart Sorensen, Andreas Toft Klapoetke, Nathan Cao Matthews, Gillian A. Tye, Kay Noninvasive optical inhibition with a red-shifted microbial rhodopsin |
| title | Noninvasive optical inhibition with a red-shifted microbial rhodopsin |
| title_full | Noninvasive optical inhibition with a red-shifted microbial rhodopsin |
| title_fullStr | Noninvasive optical inhibition with a red-shifted microbial rhodopsin |
| title_full_unstemmed | Noninvasive optical inhibition with a red-shifted microbial rhodopsin |
| title_short | Noninvasive optical inhibition with a red-shifted microbial rhodopsin |
| title_sort | noninvasive optical inhibition with a red shifted microbial rhodopsin |
| url | http://hdl.handle.net/1721.1/95967 https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0001-6754-0333 https://orcid.org/0000-0002-7002-1275 https://orcid.org/0000-0002-5680-2630 https://orcid.org/0000-0002-5534-6020 https://orcid.org/0000-0001-9969-4281 https://orcid.org/0000-0002-0419-3351 |
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