Poly(A)-tail profiling reveals an embryonic switch in translational control
Poly(A) tails enhance the stability and translation of most eukaryotic messenger RNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding of poly(A)-tail function. Here we describe poly(A)-tail length profiling by sequencing (PAL-seq) and apply it to measu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2015
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| Online Access: | http://hdl.handle.net/1721.1/96227 https://orcid.org/0000-0002-6410-4699 https://orcid.org/0000-0002-3872-2856 https://orcid.org/0000-0002-4890-424X https://orcid.org/0000-0001-5029-5909 https://orcid.org/0000-0001-9051-1696 |
| _version_ | 1826201050786299904 |
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| author | Subtelny, Alexander Orest Eichhorn, Stephen William Chen, Grace Sive, Hazel L. Bartel, David |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Subtelny, Alexander Orest Eichhorn, Stephen William Chen, Grace Sive, Hazel L. Bartel, David |
| author_sort | Subtelny, Alexander Orest |
| collection | MIT |
| description | Poly(A) tails enhance the stability and translation of most eukaryotic messenger RNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding of poly(A)-tail function. Here we describe poly(A)-tail length profiling by sequencing (PAL-seq) and apply it to measure tail lengths of millions of individual RNAs isolated from yeasts, cell lines, Arabidopsis thaliana leaves, mouse liver, and zebrafish and frog embryos. Poly(A)-tail lengths were conserved between orthologous mRNAs, with mRNAs encoding ribosomal proteins and other ‘housekeeping’ proteins tending to have shorter tails. As expected, tail lengths were coupled to translational efficiencies in early zebrafish and frog embryos. However, this strong coupling diminished at gastrulation and was absent in non-embryonic samples, indicating a rapid developmental switch in the nature of translational control. This switch complements an earlier switch to zygotic transcriptional control and explains why the predominant effect of microRNA-mediated deadenylation concurrently shifts from translational repression to mRNA destabilization. |
| first_indexed | 2024-09-23T11:45:34Z |
| format | Article |
| id | mit-1721.1/96227 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:45:34Z |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/962272022-10-01T05:47:45Z Poly(A)-tail profiling reveals an embryonic switch in translational control Subtelny, Alexander Orest Eichhorn, Stephen William Chen, Grace Sive, Hazel L. Bartel, David Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biology Subtelny, Alexander Orest Eichhorn, Stephen William Chen, Grace Sive, Hazel L. Bartel, David Poly(A) tails enhance the stability and translation of most eukaryotic messenger RNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding of poly(A)-tail function. Here we describe poly(A)-tail length profiling by sequencing (PAL-seq) and apply it to measure tail lengths of millions of individual RNAs isolated from yeasts, cell lines, Arabidopsis thaliana leaves, mouse liver, and zebrafish and frog embryos. Poly(A)-tail lengths were conserved between orthologous mRNAs, with mRNAs encoding ribosomal proteins and other ‘housekeeping’ proteins tending to have shorter tails. As expected, tail lengths were coupled to translational efficiencies in early zebrafish and frog embryos. However, this strong coupling diminished at gastrulation and was absent in non-embryonic samples, indicating a rapid developmental switch in the nature of translational control. This switch complements an earlier switch to zygotic transcriptional control and explains why the predominant effect of microRNA-mediated deadenylation concurrently shifts from translational repression to mRNA destabilization. National Institutes of Health (U.S.) (Grant GM067031) National Institutes of Health (U.S.) (Medical Scientist Training Program Fellowship T32GM007753) 2015-03-27T18:08:51Z 2015-03-27T18:08:51Z 2014-01 2013-07 Article http://purl.org/eprint/type/JournalArticle 0028-0836 1476-4687 http://hdl.handle.net/1721.1/96227 Subtelny, Alexander O., Stephen W. Eichhorn, Grace R. Chen, Hazel Sive, and David P. Bartel. “Poly(A)-Tail Profiling Reveals an Embryonic Switch in Translational Control.” Nature 508, no. 7494 (January 29, 2014): 66–71. https://orcid.org/0000-0002-6410-4699 https://orcid.org/0000-0002-3872-2856 https://orcid.org/0000-0002-4890-424X https://orcid.org/0000-0001-5029-5909 https://orcid.org/0000-0001-9051-1696 en_US http://dx.doi.org/10.1038/nature13007 Nature Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Subtelny, Alexander Orest Eichhorn, Stephen William Chen, Grace Sive, Hazel L. Bartel, David Poly(A)-tail profiling reveals an embryonic switch in translational control |
| title | Poly(A)-tail profiling reveals an embryonic switch in translational control |
| title_full | Poly(A)-tail profiling reveals an embryonic switch in translational control |
| title_fullStr | Poly(A)-tail profiling reveals an embryonic switch in translational control |
| title_full_unstemmed | Poly(A)-tail profiling reveals an embryonic switch in translational control |
| title_short | Poly(A)-tail profiling reveals an embryonic switch in translational control |
| title_sort | poly a tail profiling reveals an embryonic switch in translational control |
| url | http://hdl.handle.net/1721.1/96227 https://orcid.org/0000-0002-6410-4699 https://orcid.org/0000-0002-3872-2856 https://orcid.org/0000-0002-4890-424X https://orcid.org/0000-0001-5029-5909 https://orcid.org/0000-0001-9051-1696 |
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