Computational design of a red fluorophore ligase for site-specific protein labeling in living cells
Chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherich...
Main Authors: | Baker, David, Ting, Alice Y., Liu, Daniel S., Nivon, Lucas G., Richter, Florian, Goldman, Peter J., Yao, Jennifer Z., Phipps, William S., Ye, Anne Z., Deerinck, Thomas J., Richardson, Douglas, Ellisman, Mark H., Drennan, Catherine L |
---|---|
Other Authors: | Massachusetts Institute of Technology. Department of Biology |
Format: | Article |
Language: | en_US |
Published: |
National Academy of Sciences (U.S.)
2015
|
Online Access: | http://hdl.handle.net/1721.1/96304 https://orcid.org/0000-0001-5486-2755 https://orcid.org/0000-0002-8277-5226 |
Similar Items
-
A fluorophore ligase for site-specific protein labeling inside living cells
by: Uttamapinant, Chayasith, et al.
Published: (2013) -
Structure-Guided Engineering of a Pacific Blue Fluorophore Ligase for Specific Protein Imaging in Living Cells
by: Cohen, Justin D., et al.
Published: (2012) -
Advances in molecular probe-based labeling tools and their application to multiscale multimodal correlated microscopies
by: Phan, S., et al.
Published: (2016) -
Directed evolution of APEX2 for electron microscopy and proximity labeling
by: Kamer, Kimberli J, et al.
Published: (2017) -
Labeling proteins inside living cells using external fluorophores for microscopy
by: Kai Wen Teng, et al.
Published: (2016-12-01)