Single cell sequencing reveals low levels of aneuploidy across mammalian tissues
Whole-chromosome copy number alterations, also known as aneuploidy, are associated with adverse consequences in most cells and organisms. However, high frequencies of aneuploidy have been reported to occur naturally in the mammalian liver and brain, fueling speculation that aneuploidy provides a sel...
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2015
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| Online Access: | http://hdl.handle.net/1721.1/96316 https://orcid.org/0000-0002-0989-8115 https://orcid.org/0000-0001-9837-0314 https://orcid.org/0000-0003-0649-7428 |
| _version_ | 1811097906651856896 |
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| author | Wu, Jie Whittaker, Charles A. Knouse, Kristin Ann Amon, Angelika B |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Wu, Jie Whittaker, Charles A. Knouse, Kristin Ann Amon, Angelika B |
| author_sort | Wu, Jie |
| collection | MIT |
| description | Whole-chromosome copy number alterations, also known as aneuploidy, are associated with adverse consequences in most cells and organisms. However, high frequencies of aneuploidy have been reported to occur naturally in the mammalian liver and brain, fueling speculation that aneuploidy provides a selective advantage in these organs. To explore this paradox, we used single cell sequencing to obtain a genome-wide, high-resolution assessment of chromosome copy number alterations in mouse and human tissues. We find that aneuploidy occurs much less frequently in the liver and brain than previously reported and is no more prevalent in these tissues than in skin. Our results highlight the rarity of chromosome copy number alterations across mammalian tissues and argue against a positive role for aneuploidy in organ function. Cancer is therefore the only known example, in mammals, of altering karyotype for functional adaptation. |
| first_indexed | 2024-09-23T17:06:51Z |
| format | Article |
| id | mit-1721.1/96316 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T17:06:51Z |
| publishDate | 2015 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/963162022-09-29T23:43:49Z Single cell sequencing reveals low levels of aneuploidy across mammalian tissues Wu, Jie Whittaker, Charles A. Knouse, Kristin Ann Amon, Angelika B Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Knouse, Kristin Ann Wu, Jie Whittaker, Charles A. Amon, Angelika B. Whole-chromosome copy number alterations, also known as aneuploidy, are associated with adverse consequences in most cells and organisms. However, high frequencies of aneuploidy have been reported to occur naturally in the mammalian liver and brain, fueling speculation that aneuploidy provides a selective advantage in these organs. To explore this paradox, we used single cell sequencing to obtain a genome-wide, high-resolution assessment of chromosome copy number alterations in mouse and human tissues. We find that aneuploidy occurs much less frequently in the liver and brain than previously reported and is no more prevalent in these tissues than in skin. Our results highlight the rarity of chromosome copy number alterations across mammalian tissues and argue against a positive role for aneuploidy in organ function. Cancer is therefore the only known example, in mammals, of altering karyotype for functional adaptation. National Institutes of Health (U.S.). Physical Sciences Oncology Center (Grant 5-U54-CA143874) Ellison Medical Foundation (Senior Scholar Award) National Cancer Institute (U.S.) (Koch Institute. Grant P30-CA14051) Howard Hughes Medical Institute Kathy and Curt Marble Cancer Research Fund 2015-04-01T17:37:43Z 2015-04-01T17:37:43Z 2014-09 2014-07 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/96316 Knouse, Kristin A., Jie Wu, Charles A. Whittaker, and Angelika Amon. “Single Cell Sequencing Reveals Low Levels of Aneuploidy Across Mammalian Tissues.” Proceedings of the National Academy of Sciences 111, no. 37 (September 2, 2014): 13409–13414. https://orcid.org/0000-0002-0989-8115 https://orcid.org/0000-0001-9837-0314 https://orcid.org/0000-0003-0649-7428 en_US http://dx.doi.org/10.1073/pnas.1415287111 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) National Academy of Sciences (U.S.) |
| spellingShingle | Wu, Jie Whittaker, Charles A. Knouse, Kristin Ann Amon, Angelika B Single cell sequencing reveals low levels of aneuploidy across mammalian tissues |
| title | Single cell sequencing reveals low levels of aneuploidy across mammalian tissues |
| title_full | Single cell sequencing reveals low levels of aneuploidy across mammalian tissues |
| title_fullStr | Single cell sequencing reveals low levels of aneuploidy across mammalian tissues |
| title_full_unstemmed | Single cell sequencing reveals low levels of aneuploidy across mammalian tissues |
| title_short | Single cell sequencing reveals low levels of aneuploidy across mammalian tissues |
| title_sort | single cell sequencing reveals low levels of aneuploidy across mammalian tissues |
| url | http://hdl.handle.net/1721.1/96316 https://orcid.org/0000-0002-0989-8115 https://orcid.org/0000-0001-9837-0314 https://orcid.org/0000-0003-0649-7428 |
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