SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes
S-Adenosylmethionine (SAM, also known as AdoMet) radical enzymes use SAM and a [4Fe-4S] cluster to catalyze a diverse array of reactions. They adopt a partial triose-phosphate isomerase (TIM) barrel fold with N- and C-terminal extensions that tailor the structure of the enzyme to its specific functi...
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| Format: | Article |
| Language: | en_US |
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American Society for Biochemistry and Molecular Biology (ASBMB)
2015
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| Online Access: | http://hdl.handle.net/1721.1/96728 https://orcid.org/0000-0001-5486-2755 https://orcid.org/0000-0001-8578-7559 |
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| author | Goldman, Peter J. Grell, Tsehai Ariane Drennan, Catherine L |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Goldman, Peter J. Grell, Tsehai Ariane Drennan, Catherine L |
| author_sort | Goldman, Peter J. |
| collection | MIT |
| description | S-Adenosylmethionine (SAM, also known as AdoMet) radical enzymes use SAM and a [4Fe-4S] cluster to catalyze a diverse array of reactions. They adopt a partial triose-phosphate isomerase (TIM) barrel fold with N- and C-terminal extensions that tailor the structure of the enzyme to its specific function. One extension, termed a SPASM domain, binds two auxiliary [4Fe-4S] clusters and is present within peptide-modifying enzymes. The first structure of a SPASM-containing enzyme, anaerobic sulfatase-maturating enzyme (anSME), revealed unexpected similarities to two non-SPASM proteins, butirosin biosynthetic enzyme 2-deoxy-scyllo-inosamine dehydrogenase (BtrN) and molybdenum cofactor biosynthetic enzyme (MoaA). The latter two enzymes bind one auxiliary cluster and exhibit a partial SPASM motif, coined a Twitch domain. Here we review the structure and function of auxiliary cluster domains within the SAM radical enzyme superfamily. |
| first_indexed | 2024-09-23T09:47:19Z |
| format | Article |
| id | mit-1721.1/96728 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:47:19Z |
| publishDate | 2015 |
| publisher | American Society for Biochemistry and Molecular Biology (ASBMB) |
| record_format | dspace |
| spelling | mit-1721.1/967282022-09-30T16:50:50Z SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes Goldman, Peter J. Grell, Tsehai Ariane Drennan, Catherine L Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Drennan, Catherine L. Grell, Tsehai Ariane Goldman, Peter J. Drennan, Catherine L. S-Adenosylmethionine (SAM, also known as AdoMet) radical enzymes use SAM and a [4Fe-4S] cluster to catalyze a diverse array of reactions. They adopt a partial triose-phosphate isomerase (TIM) barrel fold with N- and C-terminal extensions that tailor the structure of the enzyme to its specific function. One extension, termed a SPASM domain, binds two auxiliary [4Fe-4S] clusters and is present within peptide-modifying enzymes. The first structure of a SPASM-containing enzyme, anaerobic sulfatase-maturating enzyme (anSME), revealed unexpected similarities to two non-SPASM proteins, butirosin biosynthetic enzyme 2-deoxy-scyllo-inosamine dehydrogenase (BtrN) and molybdenum cofactor biosynthetic enzyme (MoaA). The latter two enzymes bind one auxiliary cluster and exhibit a partial SPASM motif, coined a Twitch domain. Here we review the structure and function of auxiliary cluster domains within the SAM radical enzyme superfamily. Massachusetts Institute of Technology. Office of the Dean for Graduate Education (Fellowship) National Science Foundation (U.S.) (Grant MCB-0543833) 2015-04-23T14:21:20Z 2015-04-23T14:21:20Z 2014-12 Article http://purl.org/eprint/type/JournalArticle 0021-9258 1083-351X http://hdl.handle.net/1721.1/96728 Grell, Tsehai A. J., Peter J. Goldman, and Catherine L. Drennan. “SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes.” J. Biol. Chem. 290, no. 7 (December 4, 2014): 3964–3971. https://orcid.org/0000-0001-5486-2755 https://orcid.org/0000-0001-8578-7559 en_US http://dx.doi.org/10.1074/jbc.R114.581249 Journal of Biological Chemistry Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Society for Biochemistry and Molecular Biology (ASBMB) Prof. Drennan via Erja Kajosalo |
| spellingShingle | Goldman, Peter J. Grell, Tsehai Ariane Drennan, Catherine L SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes |
| title | SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes |
| title_full | SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes |
| title_fullStr | SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes |
| title_full_unstemmed | SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes |
| title_short | SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes |
| title_sort | spasm and twitch domains in s adenosylmethionine sam radical enzymes |
| url | http://hdl.handle.net/1721.1/96728 https://orcid.org/0000-0001-5486-2755 https://orcid.org/0000-0001-8578-7559 |
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