Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling
In mouse embryos at mid-gestation, primordial germ cells (PGCs) undergo licensing to become gametogenesis-competent cells (GCCs), gaining the capacity for meiotic initiation and sexual differentiation. GCCs then initiate either oogenesis or spermatogenesis in response to gonadal cues. Germ cell lice...
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| Formato: | Artigo |
| Idioma: | en_US |
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Public Library of Science
2015
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| Acesso em linha: | http://hdl.handle.net/1721.1/96744 https://orcid.org/0000-0001-9920-3411 |
| _version_ | 1826209748756725760 |
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| author | Hu, Yueh-Chiang Nicholls, Peter K. Soh, Ying Qi Shirleen Daniele, Joseph R. Junker, Jan Philipp van Oudenaarden, Alexander Page, David C |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Hu, Yueh-Chiang Nicholls, Peter K. Soh, Ying Qi Shirleen Daniele, Joseph R. Junker, Jan Philipp van Oudenaarden, Alexander Page, David C |
| author_sort | Hu, Yueh-Chiang |
| collection | MIT |
| description | In mouse embryos at mid-gestation, primordial germ cells (PGCs) undergo licensing to become gametogenesis-competent cells (GCCs), gaining the capacity for meiotic initiation and sexual differentiation. GCCs then initiate either oogenesis or spermatogenesis in response to gonadal cues. Germ cell licensing has been considered to be a cell-autonomous and gonad-independent event, based on observations that some PGCs, having migrated not to the gonad but to the adrenal gland, nonetheless enter meiosis in a time frame parallel to ovarian germ cells -- and do so regardless of the sex of the embryo. Here we test the hypothesis that germ cell licensing is cell-autonomous by examining the fate of PGCs in Gata4 conditional mutant (Gata4 cKO) mouse embryos. Gata4, which is expressed only in somatic cells, is known to be required for genital ridge initiation. PGCs in Gata4 cKO mutants migrated to the area where the genital ridge, the precursor of the gonad, would ordinarily be formed. However, these germ cells did not undergo licensing and instead retained characteristics of PGCs. Our results indicate that licensing is not purely cell-autonomous but is induced by the somatic genital ridge. |
| first_indexed | 2024-09-23T14:28:34Z |
| format | Article |
| id | mit-1721.1/96744 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:28:34Z |
| publishDate | 2015 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/967442022-09-29T09:36:23Z Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling Hu, Yueh-Chiang Nicholls, Peter K. Soh, Ying Qi Shirleen Daniele, Joseph R. Junker, Jan Philipp van Oudenaarden, Alexander Page, David C Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Physics Whitehead Institute for Biomedical Research Hu, Yueh-Chiang Nicholls, Peter K. Soh, Ying Qi Shirleen Daniele, Joseph R. van Oudenaarden, Alexander Page, David C. In mouse embryos at mid-gestation, primordial germ cells (PGCs) undergo licensing to become gametogenesis-competent cells (GCCs), gaining the capacity for meiotic initiation and sexual differentiation. GCCs then initiate either oogenesis or spermatogenesis in response to gonadal cues. Germ cell licensing has been considered to be a cell-autonomous and gonad-independent event, based on observations that some PGCs, having migrated not to the gonad but to the adrenal gland, nonetheless enter meiosis in a time frame parallel to ovarian germ cells -- and do so regardless of the sex of the embryo. Here we test the hypothesis that germ cell licensing is cell-autonomous by examining the fate of PGCs in Gata4 conditional mutant (Gata4 cKO) mouse embryos. Gata4, which is expressed only in somatic cells, is known to be required for genital ridge initiation. PGCs in Gata4 cKO mutants migrated to the area where the genital ridge, the precursor of the gonad, would ordinarily be formed. However, these germ cells did not undergo licensing and instead retained characteristics of PGCs. Our results indicate that licensing is not purely cell-autonomous but is induced by the somatic genital ridge. National Health and Medical Research Council (Australia) (CJ Martin Fellowship, #1053776) Howard Hughes Medical Institute (Investigator) 2015-04-23T17:28:17Z 2015-04-23T17:28:17Z 2015-03 2014-08 Article http://purl.org/eprint/type/JournalArticle 1553-7404 http://hdl.handle.net/1721.1/96744 Hu, Yueh-Chiang, Peter K. Nicholls, Y. Q. Shirleen Soh, Joseph R. Daniele, Jan Philipp Junker, Alexander van Oudenaarden, and David C. Page. “Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling.” Edited by R. Scott Hawley. PLoS Genet 11, no. 3 (March 4, 2015): e1005019. https://orcid.org/0000-0001-9920-3411 en_US http://dx.doi.org/10.1371/journal.pgen.1005019 PLOS Genetics Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Hu, Yueh-Chiang Nicholls, Peter K. Soh, Ying Qi Shirleen Daniele, Joseph R. Junker, Jan Philipp van Oudenaarden, Alexander Page, David C Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling |
| title | Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling |
| title_full | Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling |
| title_fullStr | Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling |
| title_full_unstemmed | Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling |
| title_short | Licensing of Primordial Germ Cells for Gametogenesis Depends on Genital Ridge Signaling |
| title_sort | licensing of primordial germ cells for gametogenesis depends on genital ridge signaling |
| url | http://hdl.handle.net/1721.1/96744 https://orcid.org/0000-0001-9920-3411 |
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