Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness
By dividing asymmetrically, stem cells can generate two daughter cells with distinct fates. However, evidence is limited in mammalian systems for the selective apportioning of subcellular contents between daughters. We followed the fates of old and young organelles during the division of human mamma...
| Main Authors: | , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Association for the Advancement of Science (AAAS)
2015
|
| Online Access: | http://hdl.handle.net/1721.1/96748 https://orcid.org/0000-0002-0895-3557 https://orcid.org/0000-0002-7043-5013 https://orcid.org/0000-0002-1446-7256 |
| _version_ | 1811073411096510464 |
|---|---|
| author | Chen, Walter W. Marjanovic, Nemanja Zoncu, Roberto Katajisto, Pekka Dohla, Julia Chaffer, Christine L. Pentinmikko, Nalle Iqbal, Sharif Weinberg, Robert A Sabatini, David |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Chen, Walter W. Marjanovic, Nemanja Zoncu, Roberto Katajisto, Pekka Dohla, Julia Chaffer, Christine L. Pentinmikko, Nalle Iqbal, Sharif Weinberg, Robert A Sabatini, David |
| author_sort | Chen, Walter W. |
| collection | MIT |
| description | By dividing asymmetrically, stem cells can generate two daughter cells with distinct fates. However, evidence is limited in mammalian systems for the selective apportioning of subcellular contents between daughters. We followed the fates of old and young organelles during the division of human mammary stemlike cells and found that such cells apportion aged mitochondria asymmetrically between daughter cells. Daughter cells that received fewer old mitochondria maintained stem cell traits. Inhibition of mitochondrial fission disrupted both the age-dependent subcellular localization and segregation of mitochondria and caused loss of stem cell properties in the progeny cells. Hence, mechanisms exist for mammalian stemlike cells to asymmetrically sort aged and young mitochondria, and these are important for maintaining stemness properties. |
| first_indexed | 2024-09-23T09:32:38Z |
| format | Article |
| id | mit-1721.1/96748 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:32:38Z |
| publishDate | 2015 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/967482022-09-30T15:08:32Z Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness Chen, Walter W. Marjanovic, Nemanja Zoncu, Roberto Katajisto, Pekka Dohla, Julia Chaffer, Christine L. Pentinmikko, Nalle Iqbal, Sharif Weinberg, Robert A Sabatini, David Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Koch Institute for Integrative Cancer Research at MIT Chen, Walter W. Weinberg, Robert A. Sabatini, David M. Marjanovic, Nemanja Zoncu, Roberto By dividing asymmetrically, stem cells can generate two daughter cells with distinct fates. However, evidence is limited in mammalian systems for the selective apportioning of subcellular contents between daughters. We followed the fates of old and young organelles during the division of human mammary stemlike cells and found that such cells apportion aged mitochondria asymmetrically between daughter cells. Daughter cells that received fewer old mitochondria maintained stem cell traits. Inhibition of mitochondrial fission disrupted both the age-dependent subcellular localization and segregation of mitochondria and caused loss of stem cell properties in the progeny cells. Hence, mechanisms exist for mammalian stemlike cells to asymmetrically sort aged and young mitochondria, and these are important for maintaining stemness properties. National Science Foundation (U.S.). Long-Term Ecological Research Program (DEB-8811884) National Science Foundation (U.S.). Long-Term Ecological Research Program (DEB-9411972) National Science Foundation (U.S.). Long-Term Ecological Research Program (DEB-0080382) National Science Foundation (U.S.). Long-Term Ecological Research Program (DEB-0620652) National Science Foundation (U.S.). Long-Term Ecological Research Program (DEB-1234162) National Science Foundation (U.S.). (Biocomplexity Coupled Biogeocemhical Cycles. DEB-0322057) National Science Foundation (U.S.). Long-Term Research in Environmental Biology (DEB-0716587) National Science Foundation (U.S.). Long-Term Research in Environmental Biology (DEB-1242531) National Science Foundation (U.S.). Long-Term Research in Ecosystem Sciences (DEB-1120064) United States. Dept. of Energy. Program for Ecoysystem Research (DE-FG02-96ER62291) United States. Dept. of Energy. Office of Biological and Environmental Research. National Institute for Climatic Change Research (Grant DE-FC02-06ER64158) 2015-04-23T17:48:20Z 2015-04-23T17:48:20Z 2015-04 2014-08 Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 http://hdl.handle.net/1721.1/96748 Katajisto, Pekka, Julia Dohla, Christine L. Chaffer, Nalle Pentinmikko, Nemanja Marjanovic, Sharif Iqbal, Roberto Zoncu, Walter Chen, Robert A. Weinberg, and David M. Sabatini. “Asymmetric Apportioning of Aged Mitochondria Between Daughter Cells Is Required for Stemness.” Science 348, no. 6232 (April 2, 2015): 340–343. https://orcid.org/0000-0002-0895-3557 https://orcid.org/0000-0002-7043-5013 https://orcid.org/0000-0002-1446-7256 en_US http://dx.doi.org/10.1126/science.1260384 Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Chen, Walter W. Marjanovic, Nemanja Zoncu, Roberto Katajisto, Pekka Dohla, Julia Chaffer, Christine L. Pentinmikko, Nalle Iqbal, Sharif Weinberg, Robert A Sabatini, David Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness |
| title | Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness |
| title_full | Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness |
| title_fullStr | Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness |
| title_full_unstemmed | Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness |
| title_short | Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness |
| title_sort | asymmetric apportioning of aged mitochondria between daughter cells is required for stemness |
| url | http://hdl.handle.net/1721.1/96748 https://orcid.org/0000-0002-0895-3557 https://orcid.org/0000-0002-7043-5013 https://orcid.org/0000-0002-1446-7256 |
| work_keys_str_mv | AT chenwalterw asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness AT marjanovicnemanja asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness AT zoncuroberto asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness AT katajistopekka asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness AT dohlajulia asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness AT chafferchristinel asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness AT pentinmikkonalle asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness AT iqbalsharif asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness AT weinbergroberta asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness AT sabatinidavid asymmetricapportioningofagedmitochondriabetweendaughtercellsisrequiredforstemness |