Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe
The blood oxygenation level-dependent (BOLD) contrast is widely used in functional magnetic resonance imaging (fMRI) studies aimed at investigating neuronal activity. However, the BOLD signal reflects changes in blood volume and oxygenation rather than neuronal activity per se. Therefore, understand...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Society for Neuroscience
2015
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| Online Access: | http://hdl.handle.net/1721.1/98033 |
| _version_ | 1826209015493820416 |
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| author | Gagnon, Louis Sakadzic, Sava Lesage, Frederic Musacchia, Joseph J. Lefebvre, Joel Fang, Qianqian Yucel, Meryem A. Evans, Karleyton C. Mandeville, Emiri T. Cohen-Adad, Julien Polimeni, Jonathan R. Yaseen, Mohammad A. Lo, Eng H. Greve, Douglas N. Buxton, Richard B. Dale, Anders M. Devor, Anna Boas, David A. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Gagnon, Louis Sakadzic, Sava Lesage, Frederic Musacchia, Joseph J. Lefebvre, Joel Fang, Qianqian Yucel, Meryem A. Evans, Karleyton C. Mandeville, Emiri T. Cohen-Adad, Julien Polimeni, Jonathan R. Yaseen, Mohammad A. Lo, Eng H. Greve, Douglas N. Buxton, Richard B. Dale, Anders M. Devor, Anna Boas, David A. |
| author_sort | Gagnon, Louis |
| collection | MIT |
| description | The blood oxygenation level-dependent (BOLD) contrast is widely used in functional magnetic resonance imaging (fMRI) studies aimed at investigating neuronal activity. However, the BOLD signal reflects changes in blood volume and oxygenation rather than neuronal activity per se. Therefore, understanding the transformation of microscopic vascular behavior into macroscopic BOLD signals is at the foundation of physiologically informed noninvasive neuroimaging. Here, we use oxygen-sensitive two-photon microscopy to measure the BOLD-relevant microvascular physiology occurring within a typical rodent fMRI voxel and predict the BOLD signal from first principles using those measurements. The predictive power of the approach is illustrated by quantifying variations in the BOLD signal induced by the morphological folding of the human cortex. This framework is then used to quantify the contribution of individual vascular compartments and other factors to the BOLD signal for different magnet strengths and pulse sequences. |
| first_indexed | 2024-09-23T14:16:21Z |
| format | Article |
| id | mit-1721.1/98033 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:16:21Z |
| publishDate | 2015 |
| publisher | Society for Neuroscience |
| record_format | dspace |
| spelling | mit-1721.1/980332022-09-28T19:37:02Z Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe Gagnon, Louis Sakadzic, Sava Lesage, Frederic Musacchia, Joseph J. Lefebvre, Joel Fang, Qianqian Yucel, Meryem A. Evans, Karleyton C. Mandeville, Emiri T. Cohen-Adad, Julien Polimeni, Jonathan R. Yaseen, Mohammad A. Lo, Eng H. Greve, Douglas N. Buxton, Richard B. Dale, Anders M. Devor, Anna Boas, David A. Harvard University--MIT Division of Health Sciences and Technology Gagnon, Louis Boas, David A. The blood oxygenation level-dependent (BOLD) contrast is widely used in functional magnetic resonance imaging (fMRI) studies aimed at investigating neuronal activity. However, the BOLD signal reflects changes in blood volume and oxygenation rather than neuronal activity per se. Therefore, understanding the transformation of microscopic vascular behavior into macroscopic BOLD signals is at the foundation of physiologically informed noninvasive neuroimaging. Here, we use oxygen-sensitive two-photon microscopy to measure the BOLD-relevant microvascular physiology occurring within a typical rodent fMRI voxel and predict the BOLD signal from first principles using those measurements. The predictive power of the approach is illustrated by quantifying variations in the BOLD signal induced by the morphological folding of the human cortex. This framework is then used to quantify the contribution of individual vascular compartments and other factors to the BOLD signal for different magnet strengths and pulse sequences. National Institutes of Health (U.S.) (Grant P41RR14075) National Institutes of Health (U.S.) (Grant R01NS067050) National Institutes of Health (U.S.) (Grant R01NS057198) National Institutes of Health (U.S.) (Grant R01EB000790) American Heart Association (Grant 11SDG7600037) Advanced Multimodal NeuroImaging Training Program (R90DA023427) 2015-08-05T15:21:36Z 2015-08-05T15:21:36Z 2015-02 2014-12 Article http://purl.org/eprint/type/JournalArticle 0270-6474 1529-2401 http://hdl.handle.net/1721.1/98033 Gagnon, L., S. Sakad i , F. Lesage, J. J. Musacchia, J. Lefebvre, Q. Fang, M. A. Yucel, et al. “Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe.” Journal of Neuroscience 35, no. 8 (February 25, 2015): 3663–3675. en_US http://dx.doi.org/10.1523/jneurosci.3555-14.2015 Journal of Neuroscience Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Society for Neuroscience Society for Neuroscience |
| spellingShingle | Gagnon, Louis Sakadzic, Sava Lesage, Frederic Musacchia, Joseph J. Lefebvre, Joel Fang, Qianqian Yucel, Meryem A. Evans, Karleyton C. Mandeville, Emiri T. Cohen-Adad, Julien Polimeni, Jonathan R. Yaseen, Mohammad A. Lo, Eng H. Greve, Douglas N. Buxton, Richard B. Dale, Anders M. Devor, Anna Boas, David A. Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe |
| title | Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe |
| title_full | Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe |
| title_fullStr | Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe |
| title_full_unstemmed | Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe |
| title_short | Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe |
| title_sort | quantifying the microvascular origin of bold fmri from first principles with two photon microscopy and an oxygen sensitive nanoprobe |
| url | http://hdl.handle.net/1721.1/98033 |
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