Improved Gene Targeting through Cell Cycle Synchronization

Improved Gene Targeting through Cell Cycle Synchronization

Gene targeting is a challenge in organisms where non-homologous end-joining is the predominant form of recombination. We show that cell division cycle synchronization can be applied to significantly increase the rate of homologous recombination during transformation. Using hydroxyurea-mediated cell...

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Bibliographic Details
Main Authors: Tsakraklides, Vasiliki, Brevnova, Elena, Stephanopoulos, Gregory, Shaw, A. Joe
Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering
Format: Article
Language:en_US
Published: Public Library of Science 2015
Online Access:http://hdl.handle.net/1721.1/98171
https://orcid.org/0000-0001-6909-4568

Internet

http://hdl.handle.net/1721.1/98171
https://orcid.org/0000-0001-6909-4568

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