BBF RFC 94: Type IIS Assembly for Bacterial Transcriptional Units: A Standardized Assembly Method for Building Bacterial Transcriptional Units Using the Type IIS Restriction Enzymes BsaI and BbsI

BBF RFC 94: Type IIS Assembly for Bacterial Transcriptional Units: A Standardized Assembly Method for Building Bacterial Transcriptional Units Using the Type IIS Restriction Enzymes BsaI and BbsI

This RFC94 describes an assembly standard based on the Type IIS restriction enzymes BsaI and BbsI (also called BpiI). This assembly standard is based upon the Modular Cloning (MoClo) assembly strategy, which was introduced in 2011 by Weber et al. [1] and is based upon Golden Gate cloning [2]. In th...

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Bibliographic Details
Main Authors: Haddock, Traci L., Densmore, Douglas M., Appleton, Evan, Carr, Swati, Iverson, Sonya, De Freitas, Monique, Jin, Shawn, Awtry, Jake, Desai, Devina, Lozanoski, Thomas, Shah, Pooja, Agarwal, Yash, Lewis, Kathleen, Pacheco, Alan
Format: Technical Report
Language:en_US
Published: 2015
Subjects:
assembly standard
Online Access:http://hdl.handle.net/1721.1/98267

Internet

http://hdl.handle.net/1721.1/98267

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