Efficient CRISPR/Cas9-mediated genome editing in P. falciparum

Efficient CRISPR/Cas9-mediated genome editing in P. falciparum

Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmo...

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Bibliographic Details
Main Authors: Zhang, Feng, Wagner, Jeffrey C., Platt, Randall Jeffrey, Goldfless, Stephen J., Niles, Jacquin
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: Nature Publishing Group 2015
Online Access:http://hdl.handle.net/1721.1/99501
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0002-6250-8796

Internet

http://hdl.handle.net/1721.1/99501
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0002-6250-8796

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