Highly efficient Cas9-mediated transcriptional programming
The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR)...
Main Authors: | , , , , , , , , , , , , , , , , , , |
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Other Authors: | |
Format: | Article |
Language: | en_US |
Published: |
Nature Publishing Group
2015
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Online Access: | http://hdl.handle.net/1721.1/99529 https://orcid.org/0000-0003-0396-2443 https://orcid.org/0000-0003-1736-0937 https://orcid.org/0000-0002-5560-8246 |