Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose
Engineered nanomaterials are increasingly added to foods to improve quality, safety, or nutrition. Here we report the ability of ingested nanocellulose (NC) materials to reduce digestion and absorption of ingested fat. In the small intestinal phase of an acellular simulated gastrointestinal tract, t...
| Main Authors: | , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/104284 http://hdl.handle.net/10220/50214 |
| _version_ | 1811695638007513088 |
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| author | DeLoid, Glen M. Sohal, Ikjot Singh Lorente, Laura R. Molina, Ramon M. Pyrgiotakis, Georgios Stevanovic, Ana Zhang, Ruojie McClements, David Julian Geitner, Nicholas K. Bousfield, Douglas W. Demokritou, Philip Ng, Kee Woei Loo, Joachim Say Chye Bell, David C. Brain, Joseph |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering DeLoid, Glen M. Sohal, Ikjot Singh Lorente, Laura R. Molina, Ramon M. Pyrgiotakis, Georgios Stevanovic, Ana Zhang, Ruojie McClements, David Julian Geitner, Nicholas K. Bousfield, Douglas W. Demokritou, Philip Ng, Kee Woei Loo, Joachim Say Chye Bell, David C. Brain, Joseph |
| author_sort | DeLoid, Glen M. |
| collection | NTU |
| description | Engineered nanomaterials are increasingly added to foods to improve quality, safety, or nutrition. Here we report the ability of ingested nanocellulose (NC) materials to reduce digestion and absorption of ingested fat. In the small intestinal phase of an acellular simulated gastrointestinal tract, the hydrolysis of free fatty acids (FFA) from triglycerides (TG) in a high-fat food model was reduced by 48.4% when NC was added at 0.75% w/w to the food, as quantified by pH stat titration, and by 40.1% as assessed by fluorometric FFA assay. Furthermore,
translocation of TG and FFA across an in vitro cellular model of the intestinal epithelium was significantly reduced by the presence of 0.75% w/w NC in the food (TG by 52%, and FFA by 32%). Finally, in in vivo experiments, the postprandial rise in serum TG one hour after gavage with the high fat food model was reduced by 36% when 1.0% w/w NC was administered with the food. As revealed by scanning electron microscopy and molecular dynamics studies, the primary mechanisms for this effect appear to include coalescence of fat
droplets on fibrillar NC (CNF) fibers, resulting in a reduction of available surface area for lipase binding, and sequestration of bile salts, causing impaired interfacial displacement of proteins at the lipid droplet surface, and
impaired solubilization of lipid digestion products. Together these findings suggest a potential use for NC, as a food additive or supplement, to reduce absorption of ingested fat and thereby assist in weight loss and the
management of obesity. |
| first_indexed | 2024-10-01T07:26:39Z |
| format | Journal Article |
| id | ntu-10356/104284 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:26:39Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1042842023-07-14T15:55:54Z Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose DeLoid, Glen M. Sohal, Ikjot Singh Lorente, Laura R. Molina, Ramon M. Pyrgiotakis, Georgios Stevanovic, Ana Zhang, Ruojie McClements, David Julian Geitner, Nicholas K. Bousfield, Douglas W. Demokritou, Philip Ng, Kee Woei Loo, Joachim Say Chye Bell, David C. Brain, Joseph School of Materials Science & Engineering Engineering::Materials Nanocellulose Triglyceride Engineered nanomaterials are increasingly added to foods to improve quality, safety, or nutrition. Here we report the ability of ingested nanocellulose (NC) materials to reduce digestion and absorption of ingested fat. In the small intestinal phase of an acellular simulated gastrointestinal tract, the hydrolysis of free fatty acids (FFA) from triglycerides (TG) in a high-fat food model was reduced by 48.4% when NC was added at 0.75% w/w to the food, as quantified by pH stat titration, and by 40.1% as assessed by fluorometric FFA assay. Furthermore, translocation of TG and FFA across an in vitro cellular model of the intestinal epithelium was significantly reduced by the presence of 0.75% w/w NC in the food (TG by 52%, and FFA by 32%). Finally, in in vivo experiments, the postprandial rise in serum TG one hour after gavage with the high fat food model was reduced by 36% when 1.0% w/w NC was administered with the food. As revealed by scanning electron microscopy and molecular dynamics studies, the primary mechanisms for this effect appear to include coalescence of fat droplets on fibrillar NC (CNF) fibers, resulting in a reduction of available surface area for lipase binding, and sequestration of bile salts, causing impaired interfacial displacement of proteins at the lipid droplet surface, and impaired solubilization of lipid digestion products. Together these findings suggest a potential use for NC, as a food additive or supplement, to reduce absorption of ingested fat and thereby assist in weight loss and the management of obesity. Accepted version 2019-10-22T06:03:00Z 2019-12-06T21:29:48Z 2019-10-22T06:03:00Z 2019-12-06T21:29:48Z 2018 Journal Article DeLoid, G. M., Sohal, I. S., Lorente, L. R., Molina, R. M., Pyrgiotakis, G., Stevanovic, A., … Demokritou, P. (2018). Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose. ACS Nano, 12(7), 6469-6479. doi:10.1021/acsnano.8b03074 1936-0851 https://hdl.handle.net/10356/104284 http://hdl.handle.net/10220/50214 10.1021/acsnano.8b03074 en ACS Nano This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsnano.8b03074 32 p. application/pdf |
| spellingShingle | Engineering::Materials Nanocellulose Triglyceride DeLoid, Glen M. Sohal, Ikjot Singh Lorente, Laura R. Molina, Ramon M. Pyrgiotakis, Georgios Stevanovic, Ana Zhang, Ruojie McClements, David Julian Geitner, Nicholas K. Bousfield, Douglas W. Demokritou, Philip Ng, Kee Woei Loo, Joachim Say Chye Bell, David C. Brain, Joseph Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose |
| title | Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose |
| title_full | Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose |
| title_fullStr | Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose |
| title_full_unstemmed | Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose |
| title_short | Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose |
| title_sort | reducing intestinal digestion and absorption of fat using a nature derived biopolymer interference of triglyceride hydrolysis by nanocellulose |
| topic | Engineering::Materials Nanocellulose Triglyceride |
| url | https://hdl.handle.net/10356/104284 http://hdl.handle.net/10220/50214 |
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