Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells

Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells

The prokaryotic type II CRISPR/Cas9 system has been adapted to perform targeted genome editing in cells and model organisms. Here, we describe targeted gene deletion and replacement in human cells via the CRISPR/Cas9 system using two guide RNAs. The system effectively generated targeted deletions of...

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Bibliographic Details
Main Authors: Zheng, Qiupeng, Cai, Xiaohong, Tan, Meng How, Schaffert, Steven, Arnold, Christopher P., Gong, Xue, Chen, Chang-Zheng, Huang, Shenglin
Other Authors: School of Chemical and Biomedical Engineering
Format: Journal Article
Language:English
Published: 2019
Subjects:
Genome Editing
DRNTU::Engineering::Bioengineering
CRISPR/Cas9
Online Access:https://hdl.handle.net/10356/106786
http://hdl.handle.net/10220/48994

Internet

https://hdl.handle.net/10356/106786
http://hdl.handle.net/10220/48994

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