Hyaluronan receptor LYVE-1 - expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen
The maintenance of appropriate arterial tone is critically important for normal physiological arterial function. However, the cellular and molecular mechanisms remain poorly defined. Here, we have shown that in the mouse aorta, resident macrophages prevented arterial stiffness and collagen depositio...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/140056 |
| _version_ | 1826119426350514176 |
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| author | Lim, Hwee Ying Lim, Sheau Yng Tan, Chek Kun Thiam, Chung Hwee Goh, Chi Ching Carbajo, Daniel Chew, Samantha Hui Shang See, Peter Chi Ee Chakarov, Svetoslav Wang, Xiao Nong Lim, Li Hui Johnson, Louise A. Lum, Josephine Fong, Chui Yee Ariff Bongso Arijit Biswas Goh, Chern Evrard, Maximilien Yeo, Kim Pin Basu, Ranu Wang, Jun Kit Tan, Yingrou Rohit Jain Tikoo, Shweta Choong, Cleo S. N. Weninger, Wolfgang Poidinger, Michael Stanley, Richard E Collin, Matthew Tan, Nguan Soon Ng, Lai Guan Jackson, David G. Ginhoux, Florent Angeli, Véronique |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Lim, Hwee Ying Lim, Sheau Yng Tan, Chek Kun Thiam, Chung Hwee Goh, Chi Ching Carbajo, Daniel Chew, Samantha Hui Shang See, Peter Chi Ee Chakarov, Svetoslav Wang, Xiao Nong Lim, Li Hui Johnson, Louise A. Lum, Josephine Fong, Chui Yee Ariff Bongso Arijit Biswas Goh, Chern Evrard, Maximilien Yeo, Kim Pin Basu, Ranu Wang, Jun Kit Tan, Yingrou Rohit Jain Tikoo, Shweta Choong, Cleo S. N. Weninger, Wolfgang Poidinger, Michael Stanley, Richard E Collin, Matthew Tan, Nguan Soon Ng, Lai Guan Jackson, David G. Ginhoux, Florent Angeli, Véronique |
| author_sort | Lim, Hwee Ying |
| collection | NTU |
| description | The maintenance of appropriate arterial tone is critically important for normal physiological arterial function. However, the cellular and molecular mechanisms remain poorly defined. Here, we have shown that in the mouse aorta, resident macrophages prevented arterial stiffness and collagen deposition in the steady state. Using phenotyping, transcriptional profiling, and targeted deletion of Csf1r, we have demonstrated that these macrophages-which are a feature of blood vessels invested with smooth muscle cells (SMCs) in both mouse and human tissues-expressed the hyaluronan (HA) receptor LYVE-l. Furthermore, we have shown they possessed the unique ability to modulate collagen expression in SMCs by matrix metalloproteinase MMP-9-dependent proteolysis through engagement of LYVE-1 with the HA pericellular matrix of SMCs. Our study has unveiled a hitherto unknown homeostatic contribution of arterial LYVE-1+ macrophages through the control of collagen production by SMCs and has identified a function of LYVE-1 in leukocytes. |
| first_indexed | 2024-10-01T04:59:49Z |
| format | Journal Article |
| id | ntu-10356/140056 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:59:49Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1400562020-06-01T10:13:43Z Hyaluronan receptor LYVE-1 - expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen Lim, Hwee Ying Lim, Sheau Yng Tan, Chek Kun Thiam, Chung Hwee Goh, Chi Ching Carbajo, Daniel Chew, Samantha Hui Shang See, Peter Chi Ee Chakarov, Svetoslav Wang, Xiao Nong Lim, Li Hui Johnson, Louise A. Lum, Josephine Fong, Chui Yee Ariff Bongso Arijit Biswas Goh, Chern Evrard, Maximilien Yeo, Kim Pin Basu, Ranu Wang, Jun Kit Tan, Yingrou Rohit Jain Tikoo, Shweta Choong, Cleo S. N. Weninger, Wolfgang Poidinger, Michael Stanley, Richard E Collin, Matthew Tan, Nguan Soon Ng, Lai Guan Jackson, David G. Ginhoux, Florent Angeli, Véronique School of Materials Science & Engineering School of Biological Sciences Science::Biological sciences Macrophage LYVE-1 The maintenance of appropriate arterial tone is critically important for normal physiological arterial function. However, the cellular and molecular mechanisms remain poorly defined. Here, we have shown that in the mouse aorta, resident macrophages prevented arterial stiffness and collagen deposition in the steady state. Using phenotyping, transcriptional profiling, and targeted deletion of Csf1r, we have demonstrated that these macrophages-which are a feature of blood vessels invested with smooth muscle cells (SMCs) in both mouse and human tissues-expressed the hyaluronan (HA) receptor LYVE-l. Furthermore, we have shown they possessed the unique ability to modulate collagen expression in SMCs by matrix metalloproteinase MMP-9-dependent proteolysis through engagement of LYVE-1 with the HA pericellular matrix of SMCs. Our study has unveiled a hitherto unknown homeostatic contribution of arterial LYVE-1+ macrophages through the control of collagen production by SMCs and has identified a function of LYVE-1 in leukocytes. NRF (Natl Research Foundation, S’pore) NMRC (Natl Medical Research Council, S’pore) 2020-05-26T05:53:38Z 2020-05-26T05:53:38Z 2018 Journal Article Lim, H. Y., Lim, S. Y., Tan, C. K., Thiam, C. H., Goh, C. C., Carbajo, D., . . . Angeli, V. (2018). Hyaluronan receptor LYVE-1 - expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen. Immunity, 49(2), 326-341. doi:10.1016/j.immuni.2018.06.008 1074-7613 https://hdl.handle.net/10356/140056 10.1016/j.immuni.2018.06.008 30054204 2-s2.0-85051643623 2 49 326 341 en Immunity © 2018 Elsevier Inc. All rights reserved. |
| spellingShingle | Science::Biological sciences Macrophage LYVE-1 Lim, Hwee Ying Lim, Sheau Yng Tan, Chek Kun Thiam, Chung Hwee Goh, Chi Ching Carbajo, Daniel Chew, Samantha Hui Shang See, Peter Chi Ee Chakarov, Svetoslav Wang, Xiao Nong Lim, Li Hui Johnson, Louise A. Lum, Josephine Fong, Chui Yee Ariff Bongso Arijit Biswas Goh, Chern Evrard, Maximilien Yeo, Kim Pin Basu, Ranu Wang, Jun Kit Tan, Yingrou Rohit Jain Tikoo, Shweta Choong, Cleo S. N. Weninger, Wolfgang Poidinger, Michael Stanley, Richard E Collin, Matthew Tan, Nguan Soon Ng, Lai Guan Jackson, David G. Ginhoux, Florent Angeli, Véronique Hyaluronan receptor LYVE-1 - expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen |
| title | Hyaluronan receptor LYVE-1 - expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen |
| title_full | Hyaluronan receptor LYVE-1 - expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen |
| title_fullStr | Hyaluronan receptor LYVE-1 - expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen |
| title_full_unstemmed | Hyaluronan receptor LYVE-1 - expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen |
| title_short | Hyaluronan receptor LYVE-1 - expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen |
| title_sort | hyaluronan receptor lyve 1 expressing macrophages maintain arterial tone through hyaluronan mediated regulation of smooth muscle cell collagen |
| topic | Science::Biological sciences Macrophage LYVE-1 |
| url | https://hdl.handle.net/10356/140056 |
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