Optimizing Cas9-mediated HDR in HEK293

The most widely used genome editing tool is the Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated proteins (Cas) system. With its programmable guide RNA sequence for specific gene targeting, it allows for precise genome editing including substitutions, kn...

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Bibliographic Details
Main Author: Lee, Cheryl
Other Authors: Tan Meng How
Format: Final Year Project (FYP)
Language:English
Published: Nanyang Technological University 2022
Subjects:
Online Access:https://hdl.handle.net/10356/156957