Quantitative SARS-CoV-2 alpha variant B.1.1.7 tracking in wastewater by allele-specific RT-qPCR

Quantitative SARS-CoV-2 alpha variant B.1.1.7 tracking in wastewater by allele-specific RT-qPCR

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The critical need for surveillance of SARS-CoV-2 variants of concern has prompted the development of methods that can track variants in wastewater. Here, we develop and present an open-source method based on allele-specific RT-qPCR (AS RT-qPCR) that detects and quantifies the B.1.1.7 variant, target...

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Bibliographic Details
Main Authors: Lee, Wei Lin, Imakaev, Maxim, Armas, Federica, McElroy, Kyle A., Gu, Xiaoqiong, Duvallet, Claire, Chandra, Franciscus, Chen, Hongjie, Leifels, Mats, Mendola, Samuel, Floyd-O'Sullivan, Róisín, Powell, Morgan M., Wilson, Shane T., Berge, Karl L. J., Lim, Claire Y. J., Wu, Fuqing, Xiao, Amy, Moniz, Katya, Ghaeli, Newsha, Matus, Mariana, Thompson, Janelle R., Alm, Eric J.
Other Authors: Asian School of the Environment
Format: Journal Article
Language:English
Published: 2022
Subjects:
Engineering::Environmental engineering
DNA
Amplification
Online Access:https://hdl.handle.net/10356/159844

Internet

https://hdl.handle.net/10356/159844

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