Denaturants effect on E2 protein

The self-assembly of a protein cage composed of the catalytic core domain of pyruvate dehydrogenase multienzyme complex (E2) was investigated. E2 was produced in Escherichia coli (BL21 (DE3)). The hydrodynamic size of E2 was measured to be 27-28 nm using Dynamic Light Scattering technique. E2 was su...

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Bibliographic Details
Main Author: Ratna Ekawati Dharmawan
Other Authors: Lim Sierin
Format: Final Year Project (FYP)
Language:English
Published: 2009
Subjects:
Online Access:http://hdl.handle.net/10356/16871