Detection of variant of hepatitis B virus and beta-globin through DNA technology
Two systems, i) the HBV precore gene, and ii) the human 0-globin gene were used as models to develop simple, reliable and fast methods for the detection of point mutations. Three PCR-based methods were employed to detect the HBV precore stop codon mutant. They are RG-PCR, SSCP analysis and non-radio...
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| Format: | Thesis |
| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/10356/20362 |
| Summary: | Two systems, i) the HBV precore gene, and ii) the human 0-globin gene were used as models to develop simple, reliable and fast methods for the detection of point mutations. Three PCR-based methods were employed to detect the HBV precore stop codon mutant. They are RG-PCR, SSCP analysis and non-radioactive direct DNA cycle sequencing. |
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