Functional characterization of coronavirus non-structural proteins
Genetic manipulation of the RNA genomes by reverse genetics is a powerful tool to study the molecular biology and pathogenesis of RNA viruses. During construction of an infectious clone from a Vero cell-adapted coronavirus infectious bronchitis virus (IBV), we found that a G - C point mutation at nu...
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| Другие авторы: | |
| Формат: | Диссертация |
| Язык: | English |
| Опубликовано: |
2010
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| Online-ссылка: | https://hdl.handle.net/10356/39373 |
| _version_ | 1826124847652012032 |
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| author | Chen, Bo |
| author2 | James P. Tam |
| author_facet | James P. Tam Chen, Bo |
| author_sort | Chen, Bo |
| collection | NTU |
| description | Genetic manipulation of the RNA genomes by reverse genetics is a powerful tool to study the molecular biology and pathogenesis of RNA viruses. During construction of an infectious clone from a Vero cell-adapted coronavirus infectious bronchitis virus (IBV), we found that a G - C point mutation at nucleotide position 15526, causing Arg-to-Pro mutation at amino acid position 132 of the helicase protein, is lethal to the infectivity of IBV on Vero cells. When the in vitro-synthesized full-length transcripts containing this mutation were introduced into Vero cells, no infectious virus was rescued. Upon correction of the mutation, infectious virus was recovered. Further characterization of the in vitro-synthesized full-length transcripts containing the G15526C mutation demonstrated that this mutation may block the transcription of subgenomic RNAs. |
| first_indexed | 2024-10-01T06:27:02Z |
| format | Thesis |
| id | ntu-10356/39373 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:27:02Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | ntu-10356/393732023-02-28T18:43:56Z Functional characterization of coronavirus non-structural proteins Chen, Bo James P. Tam Liu Ding Xiang School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Virology Genetic manipulation of the RNA genomes by reverse genetics is a powerful tool to study the molecular biology and pathogenesis of RNA viruses. During construction of an infectious clone from a Vero cell-adapted coronavirus infectious bronchitis virus (IBV), we found that a G - C point mutation at nucleotide position 15526, causing Arg-to-Pro mutation at amino acid position 132 of the helicase protein, is lethal to the infectivity of IBV on Vero cells. When the in vitro-synthesized full-length transcripts containing this mutation were introduced into Vero cells, no infectious virus was rescued. Upon correction of the mutation, infectious virus was recovered. Further characterization of the in vitro-synthesized full-length transcripts containing the G15526C mutation demonstrated that this mutation may block the transcription of subgenomic RNAs. DOCTOR OF PHILOSOPHY (SBS) 2010-05-21T06:41:54Z 2010-05-21T06:41:54Z 2008 2008 Thesis Chen, B. (2008). Functional characterization of coronavirus non-structural proteins. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/39373 10.32657/10356/39373 en 217 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Virology Chen, Bo Functional characterization of coronavirus non-structural proteins |
| title | Functional characterization of coronavirus non-structural proteins |
| title_full | Functional characterization of coronavirus non-structural proteins |
| title_fullStr | Functional characterization of coronavirus non-structural proteins |
| title_full_unstemmed | Functional characterization of coronavirus non-structural proteins |
| title_short | Functional characterization of coronavirus non-structural proteins |
| title_sort | functional characterization of coronavirus non structural proteins |
| topic | DRNTU::Science::Biological sciences::Microbiology::Virology |
| url | https://hdl.handle.net/10356/39373 |
| work_keys_str_mv | AT chenbo functionalcharacterizationofcoronavirusnonstructuralproteins |