Immunofluorescence protects RNA signals in simultaneous RNA–DNA FISH

Cell research often requires combinational detection of RNA and DNA by fluorescence in situ hybridization (RNA–DNA FISH). However, it is difficult to preserve the fragile RNA signals through the harsh conditions used to denature the DNA template in DNA FISH. The current protocols of RNA –DNA FISH st...

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Bibliographic Details
Main Authors: Lai, Lan-Tian, Lee, Pin Jie, Zhang, Li-Feng
Other Authors: School of Biological Sciences
Format: Journal Article
Language:English
Published: 2015
Subjects:
Online Access:https://hdl.handle.net/10356/80848
http://hdl.handle.net/10220/38893