Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
Promoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2016
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| Online Access: | https://hdl.handle.net/10356/81399 http://hdl.handle.net/10220/40783 |
| _version_ | 1826111429403475968 |
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| author | Ho, Steven C. L. Yang, Yuansheng |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Ho, Steven C. L. Yang, Yuansheng |
| author_sort | Ho, Steven C. L. |
| collection | NTU |
| description | Promoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for cell engineering. As many finely controlled promoters exhibit cell and product specificity, new promoters need to be identified, optimized and carefully evaluated before use. Suitable promoters can be identified using techniques ranging from simple molecular biology methods to modern high-throughput omics screenings. Promoter engineering is often required after identification to either obtain high and sustained expression or to provide a wider range of gene expression. This review discusses some of the available methods to identify and engineer promoters for therapeutic recombinant protein expression in mammalian cells. |
| first_indexed | 2024-10-01T02:50:34Z |
| format | Journal Article |
| id | ntu-10356/81399 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:50:34Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/813992023-12-29T06:45:47Z Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells Ho, Steven C. L. Yang, Yuansheng School of Chemical and Biomedical Engineering Promoter identification Recombinant protein production Mammalian cells Promoter engineering Promoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for cell engineering. As many finely controlled promoters exhibit cell and product specificity, new promoters need to be identified, optimized and carefully evaluated before use. Suitable promoters can be identified using techniques ranging from simple molecular biology methods to modern high-throughput omics screenings. Promoter engineering is often required after identification to either obtain high and sustained expression or to provide a wider range of gene expression. This review discusses some of the available methods to identify and engineer promoters for therapeutic recombinant protein expression in mammalian cells. ASTAR (Agency for Sci., Tech. and Research, S’pore) Accepted version 2016-06-23T07:38:24Z 2019-12-06T14:30:06Z 2016-06-23T07:38:24Z 2019-12-06T14:30:06Z 2014 Journal Article Ho, S. C. L., & Yang, Y. (2014). Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells. Biotechnology Letters, 36(8), 1569-1579. 0141-5492 https://hdl.handle.net/10356/81399 http://hdl.handle.net/10220/40783 10.1007/s10529-014-1523-4 en Biotechnology Letters © 2014 Springer. This is the author created version of a work that has been peer reviewed and accepted for publication by Biotechnology Letters, Springer. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s10529-014-1523-4]. 65 p. application/pdf |
| spellingShingle | Promoter identification Recombinant protein production Mammalian cells Promoter engineering Ho, Steven C. L. Yang, Yuansheng Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells |
| title | Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells |
| title_full | Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells |
| title_fullStr | Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells |
| title_full_unstemmed | Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells |
| title_short | Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells |
| title_sort | identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells |
| topic | Promoter identification Recombinant protein production Mammalian cells Promoter engineering |
| url | https://hdl.handle.net/10356/81399 http://hdl.handle.net/10220/40783 |
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