Impact of Using Different Promoters and Matrix Attachment Regions on Recombinant Protein Expression Level and Stability in Stably Transfected CHO Cells

Impact of Using Different Promoters and Matrix Attachment Regions on Recombinant Protein Expression Level and Stability in Stably Transfected CHO Cells

High expression level and long-term expression stability are required for therapeutic protein production in mammalian cells. Three commonly used promoters from the simian virus 40 (SV40), the CHO elongation factor 1α gene (EF1α), and the human cytomegalovirus major immediate early gene (CMV) and two...

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Bibliographic Details
Main Authors: Ho, Steven C. L., Mariati, Yeo, Jessna H. M., Fang, Shiyi Goh, Yang, Yuansheng
Other Authors: School of Chemical and Biomedical Engineering
Format: Journal Article
Language:English
Published: 2016
Subjects:
Matrix attachment region (MAR)
CMV promoter
SV40 promoter
CHO elongation factor 1α promoter
Stable recombinant protein expression
CHO cells
Online Access:https://hdl.handle.net/10356/81465
http://hdl.handle.net/10220/40789

Internet

https://hdl.handle.net/10356/81465
http://hdl.handle.net/10220/40789

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