Insertion of core CpG island element into human CMV promoter for enhancing recombinant protein expression stability in CHO cells

Insertion of core CpG island element into human CMV promoter for enhancing recombinant protein expression stability in CHO cells

The human cytomegalovirus promoter (hCMV) is susceptible to gene silencing in CHO cells, most likely due to epigenetic events, such as DNA methylation and histone modifications. The core CpG island element (IE) from the hamster adenine phosphoribosyltransferase gene has been shown to prevent DNA met...

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Bibliographic Details
Main Authors: Mariati, Yeo, Jessna H. M., Koh, Esther Y. C., Ho, Steven C. L., Yang, Yuansheng
Other Authors: School of Chemical and Biomedical Engineering
Format: Journal Article
Language:English
Published: 2016
Subjects:
CHO
hCMV promoter
core CpG island element
gene silencing
expression stability
monoclonal antibody
Online Access:https://hdl.handle.net/10356/81470
http://hdl.handle.net/10220/40790

Internet

https://hdl.handle.net/10356/81470
http://hdl.handle.net/10220/40790

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