Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation

Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the...

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Dettagli Bibliografici
Autori principali: Cao, Yuan, Nguyen, Kien Truc Giang, Tam, James P., Liu, Chuan-Fa
Altri autori: School of Biological Sciences
Natura: Journal Article
Lingua:English
Pubblicazione: 2016
Soggetti:
Accesso online:https://hdl.handle.net/10356/81837
http://hdl.handle.net/10220/39726