Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation
Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the...
Autori principali: | , , , |
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Altri autori: | |
Natura: | Journal Article |
Lingua: | English |
Pubblicazione: |
2016
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Soggetti: | |
Accesso online: | https://hdl.handle.net/10356/81837 http://hdl.handle.net/10220/39726 |