Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation
Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the...
Main Authors: | Cao, Yuan, Nguyen, Kien Truc Giang, Tam, James P., Liu, Chuan-Fa |
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Other Authors: | School of Biological Sciences |
Format: | Journal Article |
Language: | English |
Published: |
2016
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Subjects: | |
Online Access: | https://hdl.handle.net/10356/81837 http://hdl.handle.net/10220/39726 |
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