Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes
It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indicat...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/83232 http://hdl.handle.net/10220/42505 |
| _version_ | 1811679208199421952 |
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| author | Zhang, Hao Li, Tianhu |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Zhang, Hao Li, Tianhu |
| author_sort | Zhang, Hao |
| collection | NTU |
| description | It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indication that negative supercoiling is present in the condensed DNA-protein complexes. Since negative supercoiling leads to formation of highly curved and compact plectonemic and toroidal DNA structures, it would be reasonable to assume that DNA negative supercoils are responsible for aggregation of histone H1-plasmidic polynucleosome complexes. |
| first_indexed | 2024-10-01T03:05:30Z |
| format | Journal Article |
| id | ntu-10356/83232 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:05:30Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/832322023-02-28T19:32:25Z Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes Zhang, Hao Li, Tianhu School of Physical and Mathematical Sciences Plasmid DNA Histone H1 It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indication that negative supercoiling is present in the condensed DNA-protein complexes. Since negative supercoiling leads to formation of highly curved and compact plectonemic and toroidal DNA structures, it would be reasonable to assume that DNA negative supercoils are responsible for aggregation of histone H1-plasmidic polynucleosome complexes. Accepted version 2017-05-26T07:04:45Z 2019-12-06T15:17:58Z 2017-05-26T07:04:45Z 2019-12-06T15:17:58Z 2017 Journal Article Zhang, H., & Li, T. (2017). Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes. Bioorganic & Medicinal Chemistry Letters, 27(2), 168-170. 0960-894X https://hdl.handle.net/10356/83232 http://hdl.handle.net/10220/42505 10.1016/j.bmcl.2016.11.088 en Bioorganic & Medicinal Chemistry Letters © 2016 Elsevier Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Bioorganic & Medicinal Chemistry Letters, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bmcl.2016.11.088]. 4 p. application/pdf |
| spellingShingle | Plasmid DNA Histone H1 Zhang, Hao Li, Tianhu Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes |
| title | Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes |
| title_full | Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes |
| title_fullStr | Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes |
| title_full_unstemmed | Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes |
| title_short | Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes |
| title_sort | presence of negative supercoiling in aggregates of histone h1 plasmidic polynucleosome complexes |
| topic | Plasmid DNA Histone H1 |
| url | https://hdl.handle.net/10356/83232 http://hdl.handle.net/10220/42505 |
| work_keys_str_mv | AT zhanghao presenceofnegativesupercoilinginaggregatesofhistoneh1plasmidicpolynucleosomecomplexes AT litianhu presenceofnegativesupercoilinginaggregatesofhistoneh1plasmidicpolynucleosomecomplexes |