Butelase-mediated cyclization and ligation of peptides and proteins

Enzymes that catalyze efficient macrocyclization or site-specific ligation of peptides and proteins can enable tools for drug design and protein engineering. Here we describe a protocol to use butelase 1, a recently discovered peptide ligase, for high-efficiency cyclization and ligation of peptides...

Full description

Bibliographic Details
Main Authors: Qiu, Yibo, Nguyen, Giang Kien Truc, Cao, Yuan, Hemu, Xinya, Liu, Chuan-Fa, Tam, James Pingkwan
Other Authors: School of Biological Sciences
Format: Journal Article
Language:English
Published: 2016
Subjects:
Online Access:https://hdl.handle.net/10356/84599
http://hdl.handle.net/10220/41879