Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila
In metazoan germline, Piwi-interacting RNAs (piRNAs) provide defence against transposons. Piwi–piRNA complex mediates transcriptional silencing of transposons in nucleus. Heterochromatin protein 1a (HP1a) has been proposed to function downstream of Piwi-piRNA complex in Drosophila. Here we show that...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/87041 http://hdl.handle.net/10220/45294 |
| _version_ | 1811677760069828608 |
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| author | Teo, Ryan Yee Wei Anand, Amit Sridhar, Vishweshwaren Okamura, Katsutomo Kai, Toshie |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Teo, Ryan Yee Wei Anand, Amit Sridhar, Vishweshwaren Okamura, Katsutomo Kai, Toshie |
| author_sort | Teo, Ryan Yee Wei |
| collection | NTU |
| description | In metazoan germline, Piwi-interacting RNAs (piRNAs) provide defence against transposons. Piwi–piRNA complex mediates transcriptional silencing of transposons in nucleus. Heterochromatin protein 1a (HP1a) has been proposed to function downstream of Piwi-piRNA complex in Drosophila. Here we show that HP1a germline knockdown (HP1a-GLKD) leads to a reduction in the total and Piwi-bound piRNAs mapping to clusters and transposons insertions, predominantly in the regions close to telomeres and centromeres, resulting in derepression of a limited number of transposons from these regions. In addition, HP1a-GLKD increases the splicing of transcripts arising from clusters in above regions, suggesting HP1a also functions upstream to piRNA processing. Evolutionarily old transposons enriched in the pericentric regions exhibit significant loss in piRNAs targeting these transposons upon HP1a-GLKD. Our study suggests that HP1a functions to repress transposons in a chromosomal compartmentalised manner. |
| first_indexed | 2024-10-01T02:42:29Z |
| format | Journal Article |
| id | ntu-10356/87041 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:42:29Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/870412023-02-28T17:02:15Z Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila Teo, Ryan Yee Wei Anand, Amit Sridhar, Vishweshwaren Okamura, Katsutomo Kai, Toshie School of Biological Sciences Heterochromatin Protein 1a Drosophila In metazoan germline, Piwi-interacting RNAs (piRNAs) provide defence against transposons. Piwi–piRNA complex mediates transcriptional silencing of transposons in nucleus. Heterochromatin protein 1a (HP1a) has been proposed to function downstream of Piwi-piRNA complex in Drosophila. Here we show that HP1a germline knockdown (HP1a-GLKD) leads to a reduction in the total and Piwi-bound piRNAs mapping to clusters and transposons insertions, predominantly in the regions close to telomeres and centromeres, resulting in derepression of a limited number of transposons from these regions. In addition, HP1a-GLKD increases the splicing of transcripts arising from clusters in above regions, suggesting HP1a also functions upstream to piRNA processing. Evolutionarily old transposons enriched in the pericentric regions exhibit significant loss in piRNAs targeting these transposons upon HP1a-GLKD. Our study suggests that HP1a functions to repress transposons in a chromosomal compartmentalised manner. NRF (Natl Research Foundation, S’pore) Published version 2018-07-27T03:53:01Z 2019-12-06T16:33:50Z 2018-07-27T03:53:01Z 2019-12-06T16:33:50Z 2018 Journal Article Teo, R. Y. W., Anand, A., Sridhar, V., Okamura, K., & Kai, T. (2018). Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila. Nature Communications, 9(1), 1735-. https://hdl.handle.net/10356/87041 http://hdl.handle.net/10220/45294 10.1038/s41467-018-03908-3 en Nature Communications © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 12 p. application/pdf |
| spellingShingle | Heterochromatin Protein 1a Drosophila Teo, Ryan Yee Wei Anand, Amit Sridhar, Vishweshwaren Okamura, Katsutomo Kai, Toshie Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila |
| title | Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila |
| title_full | Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila |
| title_fullStr | Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila |
| title_full_unstemmed | Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila |
| title_short | Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila |
| title_sort | heterochromatin protein 1a functions for pirna biogenesis predominantly from pericentric and telomeric regions in drosophila |
| topic | Heterochromatin Protein 1a Drosophila |
| url | https://hdl.handle.net/10356/87041 http://hdl.handle.net/10220/45294 |
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